Carrow, James K.

Dolatshahi-Pirouz, Alireza

Gaharwar, Akhilesh K.

Gentry, James

Jaiswal, Manish K.

Peak, Charles W.

Thakur, Ashish

Injectable hydrogels are investigated for cell encapsulation and delivery as they can shield cells from high shear forces. One of the approaches to obtain injectable hydrogels is to reinforce polymeric networks with high aspect ratio nanoparticles such as two-dimensional (2D) nanomaterials. 2D nanomaterials are an emerging class of ultrathin materials with a high degree of anisotropy and they strongly interact with polymers resulting in the formation of shear-thinning hydrogels. Here, we present 2D nanosilicate reinforced kappa-carrageenan (κCA) hydrogels for cellular delivery. κCA is a natural polysaccharide that resembles native glycosaminoglycans and can form brittle hydrogels via ionic crosslinking. The chemical modification of κCA with photocrosslinkable methacrylate groups renders the formation of a covalently crosslinked network (MκCA). Reinforcing the MκCA with 2D nanosilicates results in shear-thinning characteristics, and enhanced mechanical stiffness, elastomeric properties, and physiological stability. The shear-thinning characteristics of nanocomposite hydrogels are investigated for human mesenchymal stem cell (hMSC) delivery. The hMSCs showed high cell viability after injection and encapsulated cells showed a circular morphology. The proposed shear-thinning nanoengineered hydrogels can be used for cell delivery for cartilage tissue regeneration and 3D bioprinting

Online Research Database In Technology

Injectable shear-thinning nanoengineered hydrogels for stem cell delivery - DTU Orbit(09/11/2017) Injectable shear-thinning nanoengineered hydrogels for stem cell deliveryInjectable hydrogels are investigated for cell encapsulation and delivery as they can shield cells from high shear forces.One of the approaches to obtain injectable hydrogels is to reinforce polymeric networks with high aspect rationanoparticles such as two-dimensional (2D) nanomaterials. 2D nanomaterials are an emerging class of ultrathin materialswith a high degree of anisotropy and they strongly interact with polymers resulting in the formation of shear-thinninghydrogels. Here, we present 2D nanosilicate reinforced kappa-carrageenan (κCA) hydrogels for cellular delivery. κCA is anatural polysaccharide that resembles native glycosaminoglycans and can form brittle hydrogels via ionic crosslinking. Thechemical modification of κCA with photocrosslinkable methacrylate groups renders the formation of a covalentlycrosslinked network (MκCA). Reinforcing the MκCA with 2D nanosilicates results in shear-thinning characteristics, andenhanced mechanical stiffness, elastomeric properties, and physiological stability. The shear-thinning characteristics ofnanocomposite hydrogels are investigated for human mesenchymal stem cell (hMSC) delivery. The hMSCs showed highcell viability after injection and encapsulated cells showed a circular morphology. The proposed shear-thinningnanoengineered hydrogels can be used for cell delivery for cartilage tissue regeneration and 3D bioprinting.  General informationState: PublishedOrganisations: Department of Micro- and Nanotechnology, Colloids and Biological Interfaces, Texas A&M UniversityAuthors: Thakur, A. (Ekstern), Jaiswal, M. K. (Ekstern), Peak, C. W. (Ekstern), Carrow, J. K. (Ekstern), Gentry, J. (Ekstern), Dolatshahi-Pirouz, A. (Intern), Gaharwar, A. K. (Ekstern)Pages: 12362-12372Publication date: 2016Main Research Area: Technical/natural sciences Publication informationJournal: NanoscaleVolume: 8Issue number: 24ISSN (Print): 2040-3364Ratings: BFI (2017): BFI-level 2 Web of Science (2017): Indexed Yes BFI (2016): BFI-level 2 Scopus rating (2016): CiteScore 7.46 SJR 2.769 SNIP 1.459 Web of Science (2016): Indexed yes BFI (2015): BFI-level 2 Scopus rating (2015): SJR 2.842 SNIP 1.588 CiteScore 7.97 Web of Science (2015): Indexed yes BFI (2014): BFI-level 2 Scopus rating (2014): SJR 2.651 SNIP 1.676 CiteScore 7.64 Web of Science (2014): Indexed yes BFI (2013): BFI-level 2 Scopus rating (2013): SJR 2.55 SNIP 1.469 CiteScore 6.89 ISI indexed (2013): ISI indexed yes Web of Science (2013): Indexed yes BFI (2012): BFI-level 2 Scopus rating (2012): SJR 2.761 SNIP 1.346 CiteScore 6.08 ISI indexed (2012): ISI indexed yes Web of Science (2012): Indexed yes Scopus rating (2011): SJR 2.494 SNIP 1.448 CiteScore 5.69 ISI indexed (2011): ISI indexed no Web of Science (2011): Indexed yes Scopus rating (2010): SJR 1.827 SNIP 0.62 Web of Science (2009): Indexed yes Original language: EnglishDOIs: 10.1039/c6nr02299e Source: FindItSource-ID: 2304627536Publication: Research - peer-review › Journal article – Annual report year: 2016 

Injectable shear-thinning nanoengineered hydrogels for stem cell delivery

Ashish Thakur

Manish K. Jaiswal

Charles W. Peak

James K. Carrow

James Gentry

Alireza Dolatshahi-Pirouz

Akhilesh K. Gaharwar

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Nanoscale

http://orbit.dtu.dk/en/publications/injectable-shearthinning-nanoengineered-hydrogels-for-stem-cell-delivery(be0bb42d-94de-4f90-8364-7057fcfac01f).pdf?nofollow=true&rendering=long

Injectable shear-thinning nanoengineered hydrogels for stem cell delivery

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