The ongoing pandemic of SARS-CoV-2 calls for rapid and cost-effective
methods to accurately identify infected individuals. The vast majority
of patient samples is assessed for viral RNA presence by RT-qPCR. Our
biomedical research institute, in collaboration between partner
hospitals and an accredited clinical diagnostic laboratory, established
a diagnostic testing pipeline that has reported on more than 252,000
RT-qPCR results since its commencement at the beginning of April
2020. However, due to ongoing demand and competition for critical
resources, alternative testing strategies were sought. In this work, we
present a clinically-validated procedure for high-throughput SARSCoV-2 detection by RT-LAMP in 25 minutes that is robust, reliable,
repeatable, sensitive, specific, and inexpensive