Mutation Induced by Gamma Irradiation in Coriander Seeds and Their Identification by RAPD Makers

Abstract

This study was accomplished to detect variation generated through exposure of Coriander  ( Coriandrum sativum  L.) seeds to five  gamma irradiation doses (0,25,50,100 and150 ) GY by using eighteen  RAPDs primers as molecular marker ,results indicated that  primers OPC-05 ,OPC-19,  OPA-10,OPA-15 and OPB-06 success in giving three treatments a unique fingerprint  .Molecular size of amplified ranged between  1860 bp produced by primer OPA-04  and   115  bp    produced by primer OPW-04  . Highest degree for polymorphism was   91.66 % produced by primer OPA-04. Monomorphic bands appeared in their higher value in primer OPC-09 . Primer OPA-10 gave highest number   unique bands ( reached to seven bands )      Primer OPC-19 gave highest value for amplified bands  , primer OPA-10 gave highest value for main bands, Discrimination, in addition to  primer efficiency value  produced in their high value by primer OPA-04.This study revealed RAPD markers in detection mutation induced by gamma irradiation