Comparative Evaluation of Periodontal Ligament Cell Viability of Permanent Teeth in Five Different Storage Media Followed by Simulated Avulsion Injury: an in Vitro Study

Abstract

Aim: To evaluate and compare the viability of periodontal ligament cells of avulsed teeth in five different storage media followed by simulated avulsion injury. Settings and Design: Seventy-five premolars extracted for orthodontic therapeutic purposes were randomly and equally ivided into five groups based on storage media used [A: HBSS (control), B: Milk (experimental), C: Aloe Vera (experimental), D: Egg white (experimental), E: Coconut water (experimental)].Methods and Material: Following extractions, the teeth were placed in one of the five different storage media for 60 minutes, following which the scrapings of the PDL were collected in Falcon tubes which already contained collagenase enzyme in 2.5 mL of Phosphate buffered saline. The tubes were subsequently incubated and centrifuged. Then acquired PDL cells were stained with Trypan Blue dye and were counted under an optical microscope. Statistical Analysis Used: Results were subjected to statistical analysis using ANOVA test and Post Hoc Tukey test. p-value < 0.05 is considered to be significant. Results: HBSS showed the highest percentage of viable cells (80.14%), followed by Egg white (74.01%) and Aloe Vera (73.68%). Milk and Coconut water showed the least percent of viable cells 63.20%, 63.58% respectively