The use of biological organisms for removal of
heavy-metal in situ brings down the production cost due to the lesser
pre-treatment steps in the removal process. Initial assessments of
the heavy-metal binding bacteria from wastewaters, indicates that
Bacillus and Chryseomonas sp. exhibited positive growth response
behavior when exposed to lead and cadmium [1]. This incorporation
of the heavy-metal species leads to the increase in the molecular
mass of the protein as shown in the SDS-PAGE result (Figure 1).
In this research, two metal-binding proteins of B. subtilis 1C7I and
1P3J were analyzed and optimized using DFT method to look into
the possibility of replacement of heavy metals (Cd, Cr, Hg, and Pb)
in its metal active sites