The aim of this research is to investigate significance of apple pectin in infant nutrition, their impact on selected bacteria of infant’s gastrointestinal microbiome and total antioxidant capacity by lectrochemical determination of changes in redox potential

Avdalović, Jelena

Beškoski, Vladimir

Lugonja, Nikoleta

Miletić, Srđan

Nikolić Kokić, Aleksandra

Spasić, Snežana

Stanković, Dalibor

Central Repository of the Institute of Chemistry, Technology and Metallurgy (CER)

WORLD OFMICROBiOMEPREGNANCY, BIRTH& INFANCY31 October - 2 November 2019Milan, ItalypROGRAMMEBOOKKENES-2 GROUP microbiome.kenes.com #WorldofMicrobiome #PB119WELCOMEDear Colleagues,It is our pleasure to welcome you to the First International Conference Worldof Microbiome: Pregnancy, Birth and Infancy in Milan, Italy!Changing microbiota in pregnancy has profound effects on fetal, neonataland infant health & development as well as on maternal health. Be a part ofnew breakthroughs which will better predict and treat diseases in fetuses andinfants. Join international colleagues at the first conference that brings togetherprofessionals across microbiology, immunology, ob-gyn, neonatology, pediatricsand industry, to push forward this emerging area in microbiome.This event is a unique meeting that brings together experts to advancescientific knowledge about microbiome in pregnancy and neonatal,interpret recent research and analyze real-world case-studies. Theinaugural conference aims to promote collaboration between researchers,clinicians and industry and help them overcome barriers that limit access toprevention, care and services. Whether you are a scientist, clinical researcher,part of a start-up or an established industry company, WoMPBl 2019 is youropportunity to network and discuss the latest findings and their potentialimpact with the pioneers.The Scientific Committee,Prof. Erika IsolauriProfessor of Pediatrics, Head of theDepartment of Clinical Medicine, FacultyDr. Omry KorenAssistant Professor, Azrieli Faculty ofMedicineof MedicineUniversity of TurkuFinlandDr. Samuli RautavaNeonatologist, AdjunctProfessor of ExperimentalPediatrics and ClinicalInstructor in PediatricsTurku UniversityFinland4Bar-Ilan UniversityIsraelDr. Sorina GrisaruGranovskyDirector of High-RiskPregnancy UnitShaare Zedek MedicalCenterIsraelProf. Enrico FerrazziProfessor of Obstetricsand Gynecology. Chairat IRCCS Fondazione CaGranda, PolyclinicUniversity of MilanItalyTHE 1st INTERNATIONAL CONFERENCE ONMICROBIOME AND ITS IMPACT ON MATERNAL,FETAL AND EARLY INFANCY HEALTHEXPERTS FROM:• MicrobiolOgy• Ob-gyn• Neonatology• pediatrics• Microbiology• Immunology• Nutrition• IndustrySESSION FORMATS:• Keynote lectures• Panel discussions• OllME2 Workshop• Debates• Exhibition• Abstracts presentations17+ hours ofactive learningsessions30+ Speakersfrom14 countries7+ hours ofdedicatednetworking timeOver 45Over 90accepted15+ Confirmedsponsors andexhibitorsup to 16 CMEcredits for medicalspecialistsABSTRACT TOPICS:• Pregnancy and pregnancycomplications• Birth mode• The neonatal period• Probiotics and prebioticsin pregnancy and infancy• Other#WorldofMicrobiome #PB119029 LOSS OF NPAS2 LIVER EXPRESSION DURING FETAL & NEONATALDEVELOPMENT ALTERS THE GUT MICROBIOME AT LIGHT/DARKTIMEPOINTS IN MICEM. Jochum D. Onei/. C Shove, L, Showalter, M. Hu, D. Goodspeed.P Gonzalez. Aagaard (USA.)030 THE EFFECTS OF PECTIN ON THE GROWTH AND ANTIOXIDANTPROPERTIES OF BACTERIA ISOLATED FROM INFANT'SGASTROINTESTINAL MICROBIOME - IN VITRO STUDYN. Lugonja. Avdafovic. D. Stankovic, S Miletic, S Spasic, A Niko/ic,V eeskosk' (Serbia)031 VERTICAL MICROBIOME TRANSMISSION FROM MATERNAL BREAST MILKTO THE INFANT GUT BY COMBINED CULTIVATION AND METAGENOMICAPPROACHESS. Manara (Italy). M. Selma-Roya C. Alcantara (Spain), F. Asnicar, E. Paso//i,F. Armanini. P Ferretti C/ta/y). MC. Coflado (Spain), N Segata (italy)032 CHANGES IN EARLY INTESTINAL MICROBIOTA INDUCED BY TRANSFERSOF VAGINAL, MILK-ASSOCIATED AND FECAL MICROBIOTA FROMPREGNANT MOTHERS DO NOT PROGRAM INTESTINAL MICROBIOTA INADULT RATSC. Michel AL. Pocheron. H B}//ard. G. Le Dréan, P Parnet (France)033 NUTRITION MAY MODULATE MICROBIOMA OF PRETERM NEONATESS. Miletic J Avdafov;c. N Logonja, J. Milic. A. Niko}ic Kokic, S. Soasic(Serbia)034 IMPACT OF MEDICATION, NUTRITION AND PROBIOTICS IN THE FIRSTTHREE WEEKS OF LIVE: COMPARISON OF PRETERM INFANTS' GUTMICROBIOMES IN THREE NEONATAL INTENSIVE CARE UNITSC. Neumann. C Moiss}-Eichinger, S, Kurath-Kol\er. B. Resch, B Ur/esberger,A. Trobjsch, l. K/ymiuk (Austrta)035 MATERNAL GUT AND BREAST MILK MICROBIOTA AFFECT INFANT GUTANTIBIOTIC RESISTOME AND MOBILE GENETIC ELEMENTSK. Pårnänen A. Karkman. J. Hu}tman. C Lyra, E Pso/auri, S RautavaS, Sa/minen, H Kumar (Finland), J Bengtsson-Paime (Sweden). R, Satokari(Finland). D.G J, Larsson (Sweden), V, Marko (Finland)036 MATERNAL PRE-PREGNANCY BODY MASS INDEX (BMI) IS NOTASSOCIATED WITH DIFFERENCES IN INFANT GUT MICROBIOTADIVERSITY AT APPROXIMATELY ONE MONTH POSTPARTUMN C. Fratascosta, L. Princisval. A.C. Souza-Coelho, M Batalha,A.L. Ferreira, C Benaim, A. C Cunha Figueiredo (Brazil). B. L, Williams,M Couto-Rodrtguez. C. Guo. M Knoop (USA), G. Kac (Brazil)037 FISH OIL SUPPLEMENTATION REDUCES MATERNAL DEFENSIVEINFLAMMATION AND A GUT MICROBIOME THAT PREDICTS REDUCEDIMMUNE PRIMING IN INFANTSC. Quin, DPI Vollman, S Ghosh, N. Haskey, M, Estaki, J, Pither, JA. Barnett,J.N. Michael. e. W Birnie. D.L, Gibson (Canada)261                    ____________________________________________________________________________   WORLD OF MICROBIOME: PREGNANCY, BIRTH & INFANCY CONFERENCE 2019  31 OCTOBER – 02 NOVEMBER 2019, MILAN, ITALY  ACCEPTED ABSTRACTS  ____________________________________________________________________________                        36  030  003 - The Neonatal Period  THE EFFECTS OF PECTIN ON THE GROWTH AND ANTIOXIDANT PROPERTIES OF BACTERIA ISOLATED FROM INFANT’S GASTROINTESTINAL MICROBIOME - IN VITRO STUDY N. Lugonja1, J. Avdalovic1, D. Stankovic2, S. Miletic1, S. Spasic1, A. Nikolic3, V. Beskoski4 1Institute of Chemistry- Technology and Metallurgy- University of Belgrade- Serbia, Department of Chemistry, Belgrade, Serbia 2Vinca Institute, University of Belgrade, Belgrade, Serbia 3Institute of molecular genetics and genetic engineering-  Serbia, University of Belgrade-, Belgrade, Serbia 4Faculty of Chemistry- University of Belgrade- Serbia, Department of Biochemistry, Belgrade, Serbia   Gut microbiota is composed of microorganisms located in the intestine, and plays important role in whole body homeostasis. Pectin is a prebiotic dietary fiber, mainly from apples and citruses, which affects the gut microbiota showing local intestinal and systemic effects. However, the prebiotic significance is poorly understood in regard to their fermentation profiles and redox milieu for health effects. The aim of this research is to investigate significance of apple pectin in infant nutrition, their impact on selected bacteria of infant’s gastrointestinal microbiome and total antioxidant capacity by electrochemical determination of changes in redox potential. To investigate the in vitro fermentation of pectin by the infant stool microbiota, we anaerobically incubated the selected bifidobacteria and lactobacilli, isolated from infant’s feces collected from term vaginally delivered infants, at 3 day of life, with 0.5 % pectin. The compositions of the stool microbiota samples were observed before and after 72 h of incubation time using DGGE. The changes in redox capacity of media before and after fermentation were electrochemicaly determinated by cyclic voltammetry and differential pulse voltammetry.  Pectin has positive effect on the growth of Bifidobacterium and Lactobacillus, of infant’s gastrointestinal microbiome. In vitro fermentation of pectin by probiotic bacteria selected from infant's stool showed a bifidogenic effect, decrease in pH and an increase in redox capacity of media. In vitro electrochemical antioxidant test can quickly determine the changes during fermentation of pectin and can be used to monitor biochemical effects of fermentation, and as a growth indicator of selected species.         Poster Presentation Certificate This is to certify that Nikoleta Lugonja presented an abstract titled THE EFFECTS OF PECTIN ON THE GROWTH AND ANTIOXIDANT PROPERTIES OF BACTERIA ISOLATED FROM INFANT'S GASTROINTESTINAL MICROBIOME - IN VITRO STUDY N. Lugonja 1, J. Avdalovic 1, D. Stankovic 2, S. Miletic 1, S. Spasic 1, A. Nikolic 3, V. Beskoski 4 1Institute of Chemistry- Technology and Metallurgy- University of Belgrade- Serbia, Department of Chemistry, Belgrade, Serbia;2Vinca Institute, University of Belgrade, Belgrade, Serbia;3Institute of molecular genetics and genetic engineering- Serbia, University of Belgrade-, Belgrade, Serbia;4Faculty of Chemistry- University of Belgrade- Serbia, Department of Biochemistry, Belgrade, Serbia.            

The Effects Of Pectin On The Growth And Antioxidant Properties Of Bacteria Isolated From Infant’s Gastrointestinal Microbiome - In Vitro Study

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The Effects Of Pectin On The Growth And Antioxidant Properties Of Bacteria Isolated From Infant’s Gastrointestinal Microbiome - In Vitro Study

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