Pengaruh Pemberian Jus Jambu Biji (Psidium Guajava L), terhadap Parameter Histopatologi Radang Kronik dan Ekspresi Vascular Endothelial Growth Factor: Studi Eksperimental Laboratorik pada Adjuvant-Induced Arthritis pada Tikus Wistar

Abstract

The effect of Psidium guajava juice on the histopathologic chronic inflammation parameter and expression of vascular endothelial growth factorBackground: Poliphenol compound and vitamin C in Psidium guajava L has antioxidant, antiinflammation, and antiangiogenic effect. These effects work by inhibiting cytokine, MMP, and VEGF expression. Effects in chronic inflammation histopathological parameter and angiogenic component (VEGF) is not yet known. The objective of the study was to analyze the effect of Psidium guajava L juice on histopathological parameter of chronic inflammation (the depth of cartilage destruction, amount of mononuclear cell, amount of capillary vessel) and VEGF expression in adjuvant-induced arthritis Wistar rats.Methods: It was an laboratory experimental with post test only control group design. Two groups of male Wistar rat, aged 3-4 month, were induced with CFA 0,1 ml twice. After booster on the seventh day, control group was given aquadest, and another group was given Psidium guajava L juice 9 g/15 ml per day, divided into 3 doses for 14 days. Histopathological parameter evaluation and VEGF expression was done in 10 fields view. All data were analysed by T-Test, with level of significant p≤0.05.Result: The amount of mononuclear cell (p=0.000) and capillary vessel (p=0.000) in P group is significantly fewer than K group. The amount of capillary vessel with positive VEGF expression (p=0.138) and size of depth in cartilage destruction (p=0.120) shows no significance difference between two groups. Conclusion: Psidium guajava L juice shows a lowering effect on histopathological parameter of chronic inflammation and VEGF expression in adjuvant-induced arthritis Wistar rats