Has been done a comparation about Ethanol from Fermentation White, Purple, and Orange Uwi Variety. Determination of ethanol using UV-VIS spectophotometry according to microdifusion and using Conway dish. Beer's law is obeyed maximum length at 446 nm and equality of regretion line obtained by y = -0.3765x + 0.7835. The result of validation method obtained accuration of method and good precision appliance with the correlation coefficient ( r ) -0.9983. Limit of detection 0.0546 g/dL. Limit of quantitation 0.1821 g/dL. From result determination of uwi's sampels obtained by 3.2998%; 2,6152%; 2,6947% continued for white, orange and purple uwi's samples. Rate of uwi's samples have different meaning of according to result of ANAVA test obtained by meaning value (P) smaller than α value (0,00