: Aedes spp are the main vectors of Dengue Hemorrhagic Fever virus. Vector elimination using chemical larvicides still has many disadvantage and can pollute the environment because of that there is some research that develop natural material for larvacides. One of the natural material for larvacides is citrus leaves (Citrus aurantifolia). Citrus leaves contain Limonoida a substances that toxic to mosquito larvae. Objective : This study aimed to determine the effect of larvicides citrus leaf extract in inhibiting the growth of Aedes spp. Methods : The research is using descriptive methods by an experimental laboratory. The samples used were instar III larvae of Aedes spp are taken from Malalayang Manado. The dose used is the dose that has been shown to be effective from another research that has been done before is 100mg on liter of water. Eksperiment Results :Based on the results of the observation the citrus leaf extract has mortality rate on first eksperiment 32 larvae from total of 50 larvae, the second eksperiment 34 larvae from total of 50 larvae and the third eksperiment 34 larvae from total of 50 larvae. The average mortality rate is 67% against the larvae of Aedes spp. Conclustion : Citrus leaft ekstrack has a good potential as a larvacides