'Indonesian Agency For Agricultural Research and Development (IAARD)'
Doi
Abstract
. Devy, N.F. and Hardiyanto. 2009. Regeneration Capacity of Callus-derived from Root Segmentsof Several Local Garlic Clones. The regeneration of garlic using callus as explants is usually used for breedingprogram such as genetic transformation activities. However, this method can also be used as propagation method,especially for virus-free planting maerials. The experiment was carried out at Tissue Culture Laboratory, IndonesianCitrus and Subtropical Fruit Research Institute from March 2005 to August 2006. The experiment was arranged ina factorial randomized complete design with 2 treatments and 6 replications. The callus induction was derived fromgarlic apical root segment via in vitro. The percentage of total explants that produce callus was very high (70-100%)on both medium MS+0.2 g/l CH + 1 ppm 2,4 D and MS + 1 ppm 2, 4 D + 0.1 ppm IAA. Nevertheless, it was only2 clones that gave better callus growth and regeneration responses than others, these were Lumbu Kuning andTawangmangu. On the subculture medium (MS + 1 ppm kinetin and MS + 1 ppm IAA + 10 ppm 2-ip), the percentageof rooted embryogenic callus of both Lumbu Kuning and Tawangmangu were also high, i.e. 60 and 70% respectivelywith 2-6 roots/explant. Shoots grew as a mass, with total shoots number of 5-10 per a mass. Normal micro bulbletswere produced in the next development phas
