A forensic DNA database is a computer database that contains records of DNA profiles primarily for the purpose of systematic comparison, and matching with a scene of crime sample or individual profile. Large body of reports have continued to emerge, demonstrating the extensive efficiency and effectiveness of the DNA database in assisting criminal investigations around the world. Therefore, the present study sought to assess the knowledge and perception on the need for the establishment of a forensic DNA database in Nigeria. In conducting this research, a total of 150 questionnaires were distributed around Benin City with focus on security agents, Judiciary and University students. The questionnaire comprised of three main categories: Socio-demographic characteristics, Knowledge on the use and application of forensic DNA database, and Information on the need for Forensic DNA Database for Criminal Investigation in Nigeria. In determining the level of awareness, the following responses were obtained. Of the total population: 48.67% were not sure about what forensic DNA is used for, 20.67 had no knowledge about forensic DNA database, and 30.67% demonstrated adequate knowledge on forensic DNA database. On the need for establishment of a forensic DNA, 57.33% were unsure, and 42.67% reported that there is a need for a forensic DNA database in Nigeria. In conclusion, our results demonstrated that there is an inadequate level of awareness on the prominent role of Forensic DNA database in the criminal investigation. Therefore, negatively impacting the perception on the need for the establishment of a forensic DNA database in Nigeria