Effectiveness of Steinmann Pin Coated with Gentamicin-Loaded Chitosan on Staphylococcus Epidermidis Growth

Abstract

Introduction.Implant related infection is a serious complication and difficult to manage. Antimicrobial coating on implant surface is expected to prevent infection. Gentamicin is a broad-spectrum antibiotic that has been widely applied locally. Chitosan is a biopolymer that has bio adhesive properties and can serve as a drug carrier. Materials and methods. This is an in vitro experimental study to assess the effectiveness of gentamicin-loaded chitosan-coated Steinmann pin on the growth of Staphylococcus epidermidis. Seven test groups were made, consist of five different concentration of gentamicin-loaded chitosan, chitosan coating only, and control without coating. Evaluation is done by assessing turbidity (qualitative measurement) and colony forming unit count (quantitative measurement). The experiment was repeated three times.Results.In the evaluation of turbidity level and colony forming unit count, we obtained gradual decline in line with the concentration. Gentamicin concentration of 4μg inhibits the growth of Staphylococcus epidermidis.Conclusions.This study found that gentamicin-loaded chitosan-coated Steinmann pin could inhibit Staphylococcus epidermidis growth in vitro. The most optimum concentration of gentamicin was 4 μg