The research objective was to know the influence of livers-substituted of chicken nugget on the content of vitamin A, Fe and the preferences. The design experiments of the research was completely randomized design with 5 treatments and 4 replications. The treatments are T0 = 0%, T1 = 10%, T2 = 20%, T3 = 30% and T4 = 40% respectively. The content of vitamin A and Fe were tested by UV-VIS Spectrophotometry method. The data were analyzed by ANOVA, if the treatments significant was continued by Duncan Multiple Range Test. The preferences were tested by 25 panelist. The data were analyzed by non-parametric Kruskal Wallis H-Test using SPSS software version 16.0, if the treatments significant was continued by Wilcoxon test. The average of the content of vitamin A are T0 = 8,44%, T1 = 13,13%, T2 = 16,51%, T3 = 21,02% and T4 = 23,90%; the content of ferum (Fe) are T0 = 12,06%, T1 = 10,40%, T2 = 9,13%, T3 = 6,08% and T4 = 5,42%; and the preferences are T0 = 4,12%, T1 = 3,84%, T2 = 3,24%, T3 = 3,32% and T4 = 3,16%. The analysis showed significant (P < 0.05) to the content of vitamin A, Fe and the preferences