The objective of this study was to evaluate the genotype variations of Kejobong and Ettawa Grade (EG) goats based on the blood plasm protein using gel polyacrilamide electrophoresis (PAGE). Twenty four of blood samples taken from Kejobong goat in Purbalingga regency and twenty four of blood samples taken from Ettawa Grade (EG) goat in Purworejo regency were used as materials. Data were analyzed to calculate gene frequency, individual heterozygosity and average heterozygosity. The independent t-test was performed to analyzed the significance of gene frequency, total genotype and average heterozygosity from six locus of blood plasm protein between Kejobong and Ettawa Grade (EG) goats. Result showed that locus of pre albumin (P-alb), albumin (Alb), ceruloplasmin (Cp), transferrin (Tf), post transferrin (P-tf) and amylase-I (Amy-I) on Kejobong and Ettawa Grade (EG) goats were polymorphic. The total genotype, gene frequency and average heterozygosity of six locus blood plasm protein between Kejobong and Ettawa Grade (EG) goats were not different. Average heterozygosity of Kejobong and Ettawa Grade (EG) goats was 0.423 and 0.435, respectively