Pengembangan Biakan In-vitro Plasmodium Falciparum secara Kontinu

Abstract

To support malaria research on its' serology/immunology, chemotherapy, drug sensitivity aspects etc. especially for falciparum malaria, a large amount of antigen (parasites) is needed. These antigen could not be obtained from patients in the field only. Considering this situation, attempts have been made to develop a Plasmodium falciparum continuous culture in-vitro following a method introduced by Trager and Jensen (1976). In our laboratory, the parasite grew and multiplied nicely for 60 days. During that period of cultivation, a large amount of parasites (mostly mature trophozoite and schizont stages) have been collected for antigen production. Several tubes of mostly young trophozoites stage have been preserved, it can be cultured again in the future or transported to another laboratory for further culture