Imaging- and therapeutic targets in neoplastic and musculoskeletal inflammatory diseas

Araujo, R.F. de

Baldazzi, F.

Chan, A.B.

Cruz, L.J.

Eich, C.

Jorquera, C.

Schomann, T.

Leiden University Scholary Publications

Ceramide and palmitic acid inhibit macrophage-mediated epithelial-mesenchymal transition in colorectal cancer (vol 468, pg 153, 2020)Araujo, R.F. de; Eich, C.; Jorquera, C.; Schomann, T.; Baldazzi, F.; Chan, A.B.; Cruz, L.J.CitationAraujo, R. F. de, Eich, C., Jorquera, C., Schomann, T., Baldazzi, F., Chan, A. B., & Cruz, L. J.(2020). Ceramide and palmitic acid inhibit macrophage-mediated epithelial-mesenchymaltransition in colorectal cancer (vol 468, pg 153, 2020). Molecular And CellularBiochemistry, 469(1-2), 179-180. doi:10.1007/s11010-020-03735-5 Version: Publisher's VersionLicense: Creative Commons CC BY 4.0 licenseDownloaded from: https://hdl.handle.net/1887/3184206 Note: To cite this publication please use the final published version (if applicable).Vol.:(0123456789) Molecular and Cellular Biochemistry (2020) 469:179–180 https://doi.org/10.1007/s11010-020-03735-5CORRECTIONCorrection to: Ceramide and palmitic acid inhibit macrophage-mediated epithelial–mesenchymal transition in colorectal cancerRaimundo Fernandes de Araujo Junior1,2,3,5  · Christina Eich5 · Carla Jorquera4,5 · Timo Schomann4,5 · Fabio Baldazzi4,5 · Alan B. Chan4,5 · Luis J. Cruz5Published online: 6 May 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020Correction to:  Molecular and Cellular Biochemistry (2020) 468:153–168 https ://doi.org/10.1007/s1101 0-020-03719 -5The third and fifth author’s affiliation was published incor-rectly in the original article. Also, Fig. 5 and the Acknowl-edgement section were published incorrectly. The corrected affiliation, Fig. 5 and the Acknowledgement section are pro-vided in this correction.Acknowledgements We acknowledge support by post-doctoral fellowship from Raimundo Fernandes de Araujo Junior by CAPES 88881.119850/2016 -01. RFAJ and LJC received funding from the MSCA-ITN-2015-ETN Action grant (Grant No. 777682; proposal number: 675743; project: ISPIC). TS was supported by the EU Programs H2020-MSCA-2015-RISE PRISAR [Grant No. 644373] and H2020-MSCA-2017-RISE CANCER [Grant No. 777682]. FB received funding from the EU Program H2020-MSCA-2016-RISE CHARMED [Grant No. 734684]. AC received funding from the EU Programs H2020-WIDE-SPREAD-2017-Twinning ACORN [Grant No. 807281] and H2020-WIDESPREAD-2018- Twinning SIMICA [Grant No. 852985].Publisher’s Note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.The original article can be found online at https ://doi.org/10.1007/s1101 0-020-03719 -5. * Raimundo Fernandes de Araujo Junior  araujojr.morfologia@gmail.com1 Department of Morphology, Federal University of Rio Grande do Norte, Natal, RN 59072-970, Brazil2 Post-Graduation Programme in Structural and Functional Biology, Federal University of Rio Grande do Norte, Natal, RN 59072-970, Brazil3 Post-Graduation Programme in Health Science, Federal University of Rio Grande do Norte, Natal, RN 59072-970, Brazil4 Percuros B.V., 2333 CL Leiden, The Netherlands5 Translational Nanobiomaterials and Imaging, Department of Radiology, Leiden University Medical Center, 2333 ZA Leiden, The Netherlands180 Molecular and Cellular Biochemistry (2020) 469:179–1801 3Fig. 5  Co-culture of CT-26 cells with M2-polarized tumor-associated macrophages (TAMs) increased the mesenchymal phenotype in colorectal cancer cells. a CT-26 cells were indirectly co-cultured with CM of PA- or Cer-treated (10 μM each) M2-TAMs for 48 h and analyzed by fluorescent microscopy for Cadherin-E (left, purple) and Vimentin (right, purple) expression. The cell nuclei were stained with DAPI (blue). Scale bar = 25 µm. b Quantification of the fluorescent intensity of the Cadherin-E and Vimentin labeling in CT-26 cells upon co-culture with CM of PA- or Cer-treated M2-TAMs. c MC-38 cells were directly co-cultured with M2-TAMs treated that were with PA or Cer (2.5 μM each) for 48 h and analyzed by flow cytometer for d Vimentin and e Cadherin-E expression. All p values were compared to CT-26 cells co-cultured with CM of IL-4-treated RAW 264 as well as compared to MC-38 cells directly co-cultured with IL-4-treated RAW 264 by analysis of variance and Bonferroni’s test **p < 0.01, ***p < 0.001 versus M2-TAM CM or M2-TAM

Ceramide and palmitic acid inhibit macrophage-mediated epithelial-mesenchymal transition in colorectal cancer (vol 468, pg 153, 2020)

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Ceramide and palmitic acid inhibit macrophage-mediated epithelial-mesenchymal transition in colorectal cancer (vol 468, pg 153, 2020)

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