Objective: Infertility has been known to cause serious social and emotional problems in India. Microorganisms may be a primary cause for thisinfertility. This study was carried out to know the prevalence of microorganisms in the infertility couples of a tertiary caring teaching hospital.Methods: One thousands of couples were screened for the infertility test presented at the outpatient Department of Obstetrics and Gynaecology.The suspected infertility couples were tested for microbial contamination in their reproductive sample. Simultaneously, the demographic data of allpatients were documented, and all the data were analyzed with SPSS 20 software.Result: From one thousand couples, 288 couples were found infertility symptoms, whereas 67.71% were primary infertility problems and rest weresecondary. It is due to male factor (27.08%), female factor (50%), and both (4.86%) but 18.05 were unexplained. From vaginal swabs, 186 and semen145 microbial colony were grown. However, Chi-square (χ) showed that there is no significant difference between the growth of microbial colony from semen or vaginal swap as p=0.06. Eight varieties of bacteria (387) and Candida albicans (37) were documented. 2Conclusion: With respect to older ages of the infertile couples, it is more chances to infections. Antibiotic should be prescribed to primary infertilitycouples and infections can be avoided the cross transmission during intercourse.Keywords: Primary infertility, Candida, Semen, Antibiotic, Vaginal swab
