Background: The number of completed sit-ups in a given period of time is a commonly used assessment to measure trunk endurance in tactical populations. This study investigated factorial and construct validity of three different durations of a sit-up test—30 s, 60 s, and 120 s; Methods: Anthropometric characteristics and sit-ups performed for 30, 60, and 120 s by101 (♂: n = 62 and ♀: n = 39) police students were assessed. A factorial analysis was used to determine if three test durations group together in one factor, correlation analysis determined whether the sit-up tests were associated with anthropometric measures and whether the three variations in the test duration correlated between each other, and the Fisher’s transformation determined whether these correlations differed significantly; Results: All three sit-up variations loaded together into one factor in both sexes, providing factorial validity for all three test durations. Anthropometrics were associated with sit-up tests lasting 30 s in males and 120 s in females. A sit-up test lasting 60 s correlated significantly stronger to 120 s than to the 30 s sit-up test; Conclusions: The 60 s sit-up test seems to have the highest construct validity, as it was not affected by anthropometrics, and it may be an optimal choice for its lower risk of lower back injuries. By applying the results of this study, agencies could reduce the bias that may occur during the sit-up test and reduce the risk of injury during physical fitness assessment