Effect of Urine Agitation on measurements of hydration status.

Abstract

Hypohydration can have significant implications on physiological functions of the body and has the potential to decrease level of performance. Hypohydration can lead to increased thermal and cardiovascular strain. • Athletic trainers are commonly required to attain urine specimen samples to assess athlete hydration status for weight checks and monitoring body mass losses. Unfortunately, immediate examination of urine samples is not always possible. • As the urine sample sits, visible and non-visible sedimentation develops. No current literature addresses the sedimentation of urine samples and what procedures should be performed to ensure an accurate hydration assessment.Applied Medicine and Rehabilitation.ILL-ETD-001Each participant completed informed consent and a health questionnaire • We provided participants with clean specimen cup(s) to provide a sample(s) • Within 2 hours of collection, we analyzed each sample using the osmometer, serving as the control. • We split each sample into 3 cups. Samples were labeled according to agitation type and participant number. • All samples were stored in a thermoneutral environment • After 48 hours, we agitated each sample as per the experimental condition • HS: tipped 10 times in hourglass fashion (right side up to up side down) • Vtx: placed on vortex mixer for 10 secs at 3,000 RPM • NS: samples were not disturbe