Croubels, Siska

Devreese, Mathias

Gasthuys, Elke

Millecam, Joske

Schauvliege, Stijn

van Bergen, Thomas

English

Ghent University Academic Bibliography

5 days 6 days 8 days 9 days 4 weeks 4 weeks 4 weeks 8 weeks Arrival Catheterisation Blood collection catheters Removal   Catheterisation Blood collection catheters/venepuncture Removal Blood collection venepuncture  THE CONVENTIONAL PIG AS PK/PD/TOXICITY MODEL FOR THE PEDIATRIC SUBPOPULATION: DEVELOPMENT OF URINE AND BLOOD SAMPLING STRATEGIES IN GROWING PIGLETS Contact information: Elke Gasthuys, elke.gasthuys@ugent.be; Mathias Devreese, mathias.devreese@ugent.be    The SAFE-PEDRUG project (IWT-SBO 130033) is supported by the Agency for Innovation by Science and Technology in Flanders (IWT).  Elke Gasthuys1, Thomas van Bergen2, Stijn Schauvliege2, Joske Millecam1, Siska Croubels1, Mathias Devreese1 1Department of Pharmacology, Toxicology and Biochemistry, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium,  2Department of Surgery and Anesthesiology of Domestic Animals, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium BACKGROUND METHODS RESULTS CONCLUSION ACKNOWLEDGMENTS The presented urine (urine pouches, male piglets) and blood sampling (catheterization and venepuncture, male and female piglets) techniques make it possible to easily perform PK/PD studies growing piglets. catheterization is preferred, since this is a more ethical method of collecting multiple blood samples in piglets.  n = 4 n = 4 n = 4 n = 2 n = 4 Urine collection n = 2 The piglet is considered as a valuable alternative animal model to perform preclinical pharmacokinetic (PK), pharmacodynamic (PD) and toxicity studies in the pediatric subpopulation. To be able to perform such studies, multiple blood and urine collections are required. The aim of the present study was to develop repetitive blood and urine sampling techniques in the same piglets (n = 4, 2♂/2♀) aging eight days, four and eight weeks. Blood was collected either by a surgically-placed jugular vein catheter (eight days old (n = 4), four weeks old (n = 2)) or by direct venepuncture of the jugular vein (four weeks old (n = 2), eight weeks old (n = 4)). Urine was collected in the male piglets using non-invasive urine collection pouches (eight days, four weeks and eight weeks (n = 2)).  n = 2 Bacteriological screening nose and perineum Bacteriological screening MRSA Positive 1 piglet † Blood collection Histological analysis Surgery/anesthesia Urine collection No complications All piglets survived Negligible damage Remodelling  Urine collection n = 2 Histological analysis   jugular veins  Urine collection n = 2 n = 1 n = 2 n = 3 Non invasive technique No leakages and complications Catheters functional for 48h Collections easily performed 12 time points, 1 mL 3 collections, 1 h  

The conventional pig as PK/PD/toxicity model for the pediatric subpopulation : development of urine and blood sampling strategies in growing piglets

https://biblio.ugent.be/publication/8526003/file/8526007

The conventional pig as PK/PD/toxicity model for the pediatric subpopulation : development of urine and blood sampling strategies in growing piglets

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Ghent University Academic Bibliography