CORE
🇺🇦
make metadata, not war
Services
Services overview
Explore all CORE services
Access to raw data
API
Dataset
FastSync
Content discovery
Recommender
Discovery
OAI identifiers
OAI Resolver
Managing content
Dashboard
Bespoke contracts
Consultancy services
Support us
Support us
Membership
Sponsorship
Community governance
Advisory Board
Board of supporters
Research network
About
About us
Our mission
Team
Blog
FAQs
Contact us
3D Bio-printed Scaffold-free Nerve Constructs with Human Gingiva-derived Mesenchymal Stem Cells Promote Rat Facial Nerve Regeneration
Authors
Justin C Burrell
Kacy D Cullen
+4 more
Anh D Le
Phuong D Nguyen
Shihong Shi
Qunzhou Zhang
Publication date
1 December 2018
Publisher
ScholarlyCommons
Abstract
Despite the promising neuro-regenerative capacities of stem cells, there is currently no licensed stem cell-based product in the repair and regeneration of peripheral nerve injuries. Here, we explored the potential use of human gingiva-derived mesenchymal stem cells (GMSCs) as the only cellular component in 3D bio-printed scaffold-free neural constructs that were transplantable to bridge facial nerve defects in rats. We showed that GMSCs have the propensity to aggregate into compact 3D-spheroids that could produce their own matrix. When cultured under either 2D- or 3D-collagen scaffolds, GMSC spheroids were found to be more capable of differentiating into both neuronal and Schwann-like cells than their adherent counterparts. Using a scaffold-free 3D bio-printer system, nerve constructs were printed from GMSC spheroids in the absence of exogenous scaffolds and allowed to mature in a bioreactor. In vivo transplantation of the GMSC-laden nerve constructs promoted regeneration and functional recovery when used to bridge segmental defects in rat facial nerves. Our findings suggest that GMSCs represent an easily accessible source of MSCs for 3D bio-printing of scaffold-free nervous tissue constructs with promising potential application for repair and regeneration of peripheral nerve defects. © 2018 The Author(s)
Similar works
Full text
Open in the Core reader
Download PDF
Available Versions
ScholarlyCommons@Penn
See this paper in CORE
Go to the repository landing page
Download from data provider
oai:repository.upenn.edu:denta...
Last time updated on 12/12/2022