Kazuhiko, Tsukagoshi

Masahiko, Hashimoto

Naoya, Jinno

Takayuki, Tanaka

吸光/化学発光検出器を備えたキャピラリー電気泳動装置を、分離チューブにポリテトラフルオロエチレンキャピラリーを利用してつくった。ルミノール-ミクロペルオキシダーゼ-過酸化水素の化学発光反応を使った。イソルミノールイソチオシアネート標識グリシン、グリシルグリシン、グリシルグリシルグリシンをモデル試料として調べた。これらの試料は、はじめオンキャピラリーで吸光検出され、つづいてエンドキャピラリーで化学発光検出した。吸光と化学発光のデータは、同一試料に対してオンライン上で同時に得られ、それらは移動時間を考慮することによって、簡便かつ正確に比較することができた。We developed a capillary electrophoresis system, incorporating absorption/chemiluminescence dual detection, with the use of a poly(tetrafluoroethylene) capillary as a separation tube. Chemiluminescence reaction of luminol-microperoxydase-hydrogen peroxide was adopted. Isoluminol isothiocyanate labeled-glycine, glycylglycine, and glycylglycylglycine were examined as model samples in the present system. These samples were first analyzed by absorption detection with on-capillary, followed by chemiluminescence detection with end-capillary. The absorption and chemiluminescence data were simultaneously obtained with on-line for quite the same samples, and they were easily and precisely compared with each other and discussed in terms of the migration times.application/pd

Tanaka, Takayuki

Jinno, Naoya

Hashimoto, Masahiko

Tsukagoshi, Kazuhiko

Institutional Repositories DataBase (IRDB)

Capillary electrophoresis with absorption/chemiluminescence dual detection system using a poyl(tetrafluoroethylene) separation capillary

Capillary electrophoresis with absorption/chemiluminescence dual detection system using a poyl(tetrafluoroethylene) separation capillary

Abstract

Similar works

Full text

Available Versions

Institutional Repositories DataBase (IRDB)