Kazuhiko, Tsukagoshi

Kazuma, Yoshida

Masahiko, Hashimoto

Naoya, Jinno

管径方向分配クロマトグラフィー(TRDC)システムは未修飾のオープンキャピラリーチューブと水-親水性/疎水性有機溶媒混合液をキャリア溶液として用いる。我々は化学発光検出器を備えたTRDCシステムにより硫酸銅、ヘマチン、ヘモグロビンのような金属化合物を分析した。モデル試料のヘマチンと銅(II)イオンおよびヘモグロビンと銅(II)イオンの混合液を落差法によってフューズドシリカキャピラリー内に注入した後に、水-アセトニトリル-酢酸エチルを体積比2:7:4および15:2:1で混合したキャリア溶液をマイクロシリンジポンプを用いて送液した。ヘマチンと銅(II)イオンおよびヘモグロビンと銅(II)イオンは有機溶媒過剰のキャリア溶液を用いた場合には管径方向への分配に基づき良好に分離して順に溶出し、化学発光により検出された。A tube radial distribution chromatography (TRDC) system was developed using an untreated open capillary tube and a water-hydrophilic-hydrophobic solvent mixture as a carrier solution. We examined metal compounds such as copper(II) sulfate, hematin, and hemoglobin using the TRDC system equipped with chemiluminescence detector. The model mixture solutions of hematin–copper(II), and hemoglobin– copper(II), were injected into a fused-silica capillary tube by the gravity method and subsequently delivered with the carrier solutions of water–acetonitrile–ethyl acetate (2:7:4 volume ratio) and water–acetonitrile–ethyl acetate (15:2:1 volume ratio), respectively, using a microsyringe pump. The mixture of hematin–copper(II) as well as hemoglobin–copper(II) was well separated and eluted with the organic solvent-rich carrier solution based on the tube radial distribution and detected by chemiluminescence in the present TRDC system.application/pd

Yoshida, Kazuma

Jinno, Naoya

Hashimoto, Masahiko

Tsukagoshi, Kazuhiko

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Metal compound analysis by capillary chromatography using an untreated capillary tube and water-hydrophilic-hydrophobic solvent mixture as a carrier solution

Metal compound analysis by capillary chromatography using an untreated capillary tube and water-hydrophilic-hydrophobic solvent mixture as a carrier solution

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