Effects of Low Level Laser Irradiation on Blood Plasma and blood parameters of Breast Cancer Patients

Abstract

 This study was designed to evaluate the effect of Low-level laser in vitro on Some blood parameters such as Erythrocytes Sedimentation Rate, Mean cell volume, hematocrit, and MCHC of patients with Breast Cancer by irradiated human blood plasma samples by using a green laser and comparing this effects pre- and post- irradiated at fixed power density (100mW/cm2) and wavelength (532nm) of the laser that we used. The 8 ml of fresh blood samples were collected in EDTA- tubes and separated into four equal quantities: one for the control (non-irradiated) sample and the other for irradiation by laser. The blood samples are separated by bench centrifuge, cells sediment at the bottom of the tube, and the plasma is supernatant in the tube. After separation, the plasma is irradiated by laser with different exposure times (10, 20, and 30) minutes. The blood parameters are measured using an automatic hematology analyzer, and then ESR values are measured using a Westergren method. The RBCs volume (MCV) increased while MCHC decreased, and the ESR values.     file:///C:/Users/DR.ALI/AppData/Local/Temp/msohtmlclip1/01/clip_image002.jpgPolish Journal of Medical Physics and Engineering September 2022 The Journal of Polish Society of Medical Physics Vol 28, Issue 4   ISSN 1898-0309, doi: 10.2478/pjmpe-2022-0023 </p