Baczkó István

Farkas Attila

Jost Norbert

Pintér Jenő A.

Polyák Alexandra

Topal L.

Varró András

Virág László

English

University of Szeged

 121  8th European Section Meeting of the International Academy of Cardiovascular Sciences (IACS); September 28 – October 1, 2022; Szeged, Hungary POTENTIAL ELECTROPHYSIOLOGICAL IMPACTS OF SUPRA-PHYSIOLOGICAL TESTOSTERONE LEVEL: INVESTIGATION OF THE EFFECTS OF TESTOSTERONE-UNDECANOATE ADMINISTRATION CHRONICALLY IN A LARGE ANIMAL MODEL  Leila Topal1, Jenő A. Pintér2, Alexandra Polyák3, Attila Farkas2, László Virág1,4, Norbert Jost1,4, István Baczkó1,4, András Varró1,4  1Department of Pharmacology and Pharmacotherapy, Albert Szent-Györgyi Medical School, University of Szeged, Szeged, Hungary; 2Department of Internal Medicine, Cardiology, Albert Szent-Györgyi Medical School, University of Szeged, Szeged, Hungary; 3Department of Internal Medicine, Albert Szent-Györgyi Medical School, University of Szeged, Szeged, Hungary; 4Working Group of Cardiovascular Pharmacology, Hungarian Academy of Sciences, Szeged, Hungary.  Despite the legally stipulated availability of various androgen anabolic steroids (AAS), abuse of these products causes a major public health problem among young adults. Chronic use of AASs may result in structural and functional remodeling of the heart. The aim of our study was to investigate the potential electrophysiological effects of chronic administration of testosterone-undecanoate in a large animal model in in vivo and in vitro studies. Eight male beagle dogs were randomized into control (’Cont’) and treated (’Tr’) groups (n = 4; n = 4). The latter group received 15 mg/kg testosterone-undecanoate weekly for 3 months. Blood samples were taken for monitoring testosterone levels. ECG and ECHO procedures were performed. Ventricular myocytes were enzymatically dissociated via retrograde perfusion. The transmembrane ionic currents were recorded using the whole-cell configuration of the patch-clamp technique and the action potential duration (APD) was measured by the perforated patch-clamp technique.  Testosterone level was significantly higher in the ‘Tr’ group compared to the ‘Cont’ group (47.02 nm/L vs. 15.23 nm/L; p = 0.0002). The APD of isolated left ventricular myocytes was seemingly shorter in the ‘Tr’ group. In terms of different potassium currents, the amplitude of the slow delayed rectifier potassium current was increased in the ’Tr’ group. The repolarization of canine ventricular myocardium was significantly modified by constantly high doses of testosterone. Although the physiological level of testosterone provided many benefits to health, the supra-physiological level induced potential cellular and structural changes may lead to life-threatening arrhythmias under certain circumstances.  Keywords: testosterone, electrophysiology, patch-clamp, hypertrophy, fibrosis             

Potential electrophysiological impacts of supra-physiological testosterone level : investigation of the effects of testosterone-undecanoate administration chronically in a large animal model : [abstract]

http://acta.bibl.u-szeged.hu/77581/1/iacs_es_2022_121.pdf

Potential electrophysiological impacts of supra-physiological testosterone level : investigation of the effects of testosterone-undecanoate administration chronically in a large animal model : [abstract]

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University of Szeged