Amidžić Ljiljana

Bajić Zorislava

Jovičić Sanja

Katana Maja

Stojiljković Miloš P.

Vojinović Nataša

Škrbic Ranko

Šobot Tanja

English

University of Szeged

 124  8th European Section Meeting of the International Academy of Cardiovascular Sciences (IACS); September 28 – October 1, 2022; Szeged, Hungary LIRAGLUTIDE ATTENUATES ISOPRENALINE-INDUCED MYOCARDIAL INJURY AND APOPTOSIS IN RATS BY MODULATING WNT/β-CATENIN SIGNALLING PATHWAY  Natasa Vojinovic1, Ljiljana Amidzic2, Sanja Jovicic3, Maja Katana1, Tanja Sobot4,Zorislava Bajic4, Milos P Stojiljkovic1, 5, Ranko Skrbic1, 5  1Centre for Biomedical Research, Faculty of Medicine, University of Banja Luka, 78 000 Banja Luka, the Republic of Srpska, Bosnia and Herzegovina 2Department of Human Genetics, Faculty of Medicine University of Banja Luka, 78 000 Banja Luka, the Republic of Srpska, Bosnia and Herzegovina 3Department of Histology and Embryology, Faculty of Medicine University of Banja Luka,78 000 Banja Luka, the Republic of Srpska, Bosnia and Herzegovina 4Department of Physiology, Faculty of Medicine University of Banja Luka, 78 000 Banja Luka, the Republic of Srpska, Bosnia and Herzegovina 5Department of Pharmacology, Toxicology and Clinical Pharmacology, Faculty of Medicine, University of Banja Luka, 78 000 Banja Luka, the Republic of Srpska, Bosnia and Herzegovina  β-adrenergic receptor stimulation with isoprenaline (ISO) induces substantial oxidative stress in the heart, leading to the infarct-like necrotic damage. During myocardial injury (MI) reactive oxygen species are generated, which causes cardiomyocyte apoptosis. The up-regulation of canonical Wnt/β-catenin pathway induces cardiac hypertrophy and fibrosis in the isoprenaline (ISO)-induced model of MI. The aim of this study was to clarify the role of cardioprotective effects of liraglutide (LIR), an antidiabetic glucagon-like peptide-1 (GLP-1) receptor agonist on ISO-induced myocardial pathological manifestations. The rats in this study were randomly allocated to four groups: control (C) group, I group (physiological saline + ISO injection for two days at a dose of 85 mg/kg), L group (LIR for 10 days + physiological saline) and L+I group (LIR for 10 days + ISO on days 9 and 10). In order to produce an infarct-like necrotic damage, ISO injections were given to Wistar albino rats for two days. The expression of β-catenin, cyclin D1 and cleaved caspase-3 (a key apoptotic protein) was monitored using immunohistochemical detection. The results of the present study suggest that pre-treatment with LIR significantly ameliorated ISO-induced MI in rats. It also decreased apoptotic marker expression, which subsequently resulted in the significant decrease in the expression levels of Wnt/β-catenin signalling pathway-associated molecules such as β-catenin and cyclin D1. Our findings revealed that Wnt/β-catenin signalling pathway is a potential molecular target for LIR, what might be promising in the prevention of MI and apoptosis in cardiomyocytes.  Keywords: Liraglutide, Cardioprotection, Wnt/β-catenin, Cleaved caspase-3, Isoprenaline induced myocardial injury            

Liraglutide attenuates isoprenaline-induced myocardial injury and apoptosis in rats by modulating WNT/β-Catenin signalling pathway : [abstract]

http://acta.bibl.u-szeged.hu/77584/1/iacs_es_2022_124.pdf

Liraglutide attenuates isoprenaline-induced myocardial injury and apoptosis in rats by modulating WNT/β-Catenin signalling pathway : [abstract]

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