Jakovljevic Vladimir

Mihajlovic Katarina

Mikhalyeva Anastasia

Nikolic Turnic Tamara

Pindovic Bozidar

Rankovic M.

Srejovic Ivan

Zivkovic Vladimir

English

University of Szeged

 110  8th European Section Meeting of the International Academy of Cardiovascular Sciences (IACS); September 28 – October 1, 2022; Szeged, Hungary THE ROLE OF INSULIN TREATMENT IN CONTROLLING OXIDATIVE STRESS IN BLOOD OF DIABETIC RATS  Tamara Nikolic Turnic1,2, Bozidar Pindovic1, Katarina Mihajlovic1, Marina Rankovic3, Mikhalyeva Anastasia4, Ivan Srejovic3,5, Vladimir Zivkovic3,5, Vladimir Jakovljevic3,4  1Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, Serbia 2N.A.Semashko Public Health and Healthcare Department, F.F. Erismann Institute of Public Health, I.M. Sechenov First Moscow State Medical University, Moscow, Russia 3Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, Serbia 4Department of Human Pathology, 1st Moscow State Medical, University IM Sechenov, Moscow, Russia 5University IM Sechenov, Moscow, Russia  An increase in oxidative stress is associated with hyperglycemia, development, and progression of diabetes complications. This condition can lead to lipid peroxidation in muscle cell membranes, which contributes to the development of insulin resistance. The purpose of the present study was to verify the action of insulin combined with HBO treatment in oxidative stress control of streptozotocin-diabetic rats. The rats were subjected to 24-hour starvation and given an intraperitoneal injection of streptozotocin (STZ, 60 mg/kg body weight, dissolved in 0.01 M sodium citrate buffer, pH 4.5) to induce diabetes. A total of 48 male Wistar rats were randomly divided into 4 groups: 1) Control group, no diabetic induction without HBO treatment; 2) HBO group, exposed to 100% oxygen at 2.8 ATA (atmosphere absolute) for 1 h once daily, for 5 days (two weeks); 3) DM group, diabetes induced by streptozotocin (STZ) injection; and 4) DM + HBO group, received both STZ injection and HBO exposure; 5) DM+INS group, NPH insulin 5U/day, 6) DM+HBO+INS, received both NPH insulin and HBO exposure for 2 weeks. The body weight, glycemic control, and parameters of oxidative stress were evaluated. DM+INS reduced the oxidative stress levels and the activity of catalase and superoxide dismutase in blood when compared to DM+INS+HBO rats.  In conclusion, our results reveal that dual therapy with HBO and insulin promotes more benefits to oxidative stress control in blood of hypoinsulinemic rats than insulinotherapy alone.   Keywords: diabetes mellitus type 1, streptozotocin, hyperbaric oxygen therapy, neutral protamine hagedorn (NPH) insulin             

The role of insulin treatment in controlling oxidative stress in blood of diabetic rats : [abstract]

http://acta.bibl.u-szeged.hu/77570/1/iacs_es_2022_110.pdf

The role of insulin treatment in controlling oxidative stress in blood of diabetic rats : [abstract]

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