Cacanyiova Sona

Kalocayova Barbora

Kluknavsky Michal

Kura Branislav

Pobijakova Margita

Slezak Jan

Sykora Matus

Zorad Stefan

English

University of Szeged

 36  8th European Section Meeting of the International Academy of Cardiovascular Sciences (IACS); September 28 – October 1, 2022; Szeged, Hungary THERAPEUTIC EFFECT OF MOLECULAR HYDROGEN ON RADIATION-INDUCED OVERPRODUCTION OF FREE RADICALS IN MYOCARDIUM  Branislav Kura1, Barbora Kalocayova1, Matus Sykora1, Michal Kluknavsky2, Sona Cacanyiova2, Stefan Zorad3, Margita Pobijakova4, Jan Slezak1  1Centre of Experimental Medicine, Institute for Heart Research, Slovak Academy of Sciences, Bratislava, Slovakia 2Centre of Experimental Medicine, Institute of Normal and Pathological Physiology, Slovak Academy of Sciences, Bratislava, Slovakia 3Institute of Experimental Endocrinology, Biomedical Research Center, Department of Endocrine Regulations and Psychofarmacology, Slovak Academy of Sciences, Bratislava, Slovakia 4Department of Radiation Oncology, National Cancer Institute, Bratislava, Slovakia  Overproduction of free radicals and subsequent formation of oxidative stress is a common denominator of many cardiovascular diseases. Ionizing radiation (IR) is widely used in medicine, mostly in radiotherapy or various imaging technics. The radiation induces cells damage mostly through the formation of aggressive hydroxyl radicals. In the last 15 years, many researchers paid attention to molecular hydrogen (H2) as an effective and selective scavenger of free radicals, and as potentially therapeutic substance in many diseases. The main goal of this study was to examine a potential therapeutic effect of H2 against free radicals produced by IR directly applied to the mediastinum area of rats. The study also aimed to compare the effectiveness of two different methods of H2 administration – hydrogen-rich water (HRW) and inhalation. Rats (male, 3 months old) were irradiated with a single dose of 10 Gy. These were treated by HRW (2 mg/L) or inhalation (30 min. of 4% H2 gas) daily three times for two and nine days. The inhalation of H2 and HRW administration significantly improved all measured blood biochemical parameters (lactate dehydrogenase, alanine aminotransferase, uric acid, etc) where inhalation proved more effective. Also, irradiation-induced increased levels of selected markers of oxidative stress (superoxide dismutase, catalase, malondialdehyde) and inflammation (TNF-α and NF-κB) were significantly downregulated after H2 treatment, while inhalation was more effective here as well. Based on these results, it could be concluded that hydrogen is a protective therapeutic substance against irradiation-induced heart damage and that inhalation is more effective than drinking hydrogen-rich water.  Keywords: heart, inhalation, irradiation, molecular hydrogen, oxidative stress  This work was supported by Slovak national grants APVV-19-0317, APVV-15-0376, 2018/7838:1-26C0, 2019/4-CEMSAV1, VEGA 2/0063/18, VEGA 2/0092/22 and VEGA 2/0148/22.          

Therapeutic effect of molecular hydrogen on radiation-induced overproduction of free radicals in myocardium : [abstract]

http://acta.bibl.u-szeged.hu/77498/1/iacs_es_2022_036.pdf

Therapeutic effect of molecular hydrogen on radiation-induced overproduction of free radicals in myocardium : [abstract]

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