Bálint Tímea

Ferdinándy Péter

Korkmaz-Icöz Sevil

Merkely Béla

Nagy Dávid

Oláh Attila

Radovits Tamás

Ruppert Mihály

Sayour Alex Ali

Szabó Gábor

Ágg Bence

English

University of Szeged

52 8th European Section Meeting of the International Academy of Cardiovascular Sciences (IACS); September 28 – October 1, 2022; Szeged, Hungary THE DECOMPENSATION OF SYSTOLIC FUNCTION IN PRESSURE OVERLOAD-INDUCED LEFT VENTRICULAR MYOCARDIAL HYPERTROPHY IS ASSOCIATED WITH UNIQUE MICRORNA EXPRESSION PROFILE Dávid Nagy1, Mihály Ruppert1, Sevil Korkmaz-Icöz2, Bence Ágg3, Alex Ali Sayour1, Attila Oláh1, Tímea Bálint1, Péter Ferdinandy3, Béla Merkely1, Gábor Szabó2,4, Tamás Radovits11Heart and Vascular Centre, Semmelweis University, Budapest, Hungary 2Department of Cardiac Surgery, University of Heidelberg, Heidelberg, Germany 3Department of Pharmacology and Pharmacotherapy, Semmelweis University, Budapest 4Department of Cardiac Surgery, University Hospital Halle (Saale), Halle, Germany. MicroRNA (miRNA) expression is dysregulated in pressure overload (PO)-induced left ventricular (LV) myocardial hypertrophy (LVH). However, whether the altered miRNA expression might contribute to the decompensation of systolic function in PO-induced LVH is still debated. Thus, we aimed to characterize miRNA expression in PO-induced LVH with and without systolic heart failure (HF).  Aortic banding (AB) was performed in male rats to induce PO. Sham-operated animals served as controls. Functional and morphological alterations were assessed by echocardiography and histology. At the end of the experimental period, rats in the AB group were subcategorized based on ejection fraction [EF] into ABLVH (EF>40%) and ABHF groups (EF<40%). Global miRNA expression profiling was performed using next generation sequencing. Bioinformatic network analysis was carried out to predict miRNA-target interactions. Expression of selected target genes was measured by qRT-PCR. Increased heart weight-to-tibial length, LV mass and fibrosis confirmed the development of pathological LVH in both ABLVH and ABHF groups. Nevertheless, increased lung weight-to-tibial length, chamber dilatation and impaired EF were noted only in the ABHF group, when compared to controls. 50 miRNA showed different expression in the ABHF compared to the ABLVH group. Based on this altered expression profile, bioinformatic analysis predicted over 3000 target genes. 15 genes with high regulation strength were selected for target validation. Fmr1, Zfpm2, Wasl, Ets1 and Atg16l1 showed decreased mRNA expression levels in the ABHF group versus ABLVH. Decompensation of systolic function in PO-induced LVH is associated with unique miRNA profile leading to differential gene regulation. Keywords: heart failure; decompensation; pressure overload-induced left ventricular hypertrophy; microRNA; bioinformatic network analysis  

The decompensation of systolic function in pressure overload-induced left ventricular myocardial hypertrophy is associated with unique microRNA expression profile : [abstract]

http://acta.bibl.u-szeged.hu/77514/1/iacs_es_2022_052.pdf

The decompensation of systolic function in pressure overload-induced left ventricular myocardial hypertrophy is associated with unique microRNA expression profile : [abstract]

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University of Szeged