Frimmel Karel

Gasparovic Ivo

Hudec Vladan

Hulman Michal

Kalocayova Barbora

Kura Branislav

Luptak Jaroslav

Ondrusek Matej

Slezak Jan

Snurikova Denisa

Sramaty Rastislav

Vlkovicova Jana

Vrbjar Norbert

English

University of Szeged

 31  8th European Section Meeting of the International Academy of Cardiovascular Sciences (IACS); September 28 – October 1, 2022; Szeged, Hungary MOLECULAR HYDROGEN: NEW PROTECTIVE TOOL AGAINST ACUTE KIDNEY INJURY ASSOCIATED WITH CARDIAC SURGERY  Barbora Kalocayova1, Branislav Kura1, Jana Vlkovicova1, Denisa Snurikova1, Norbert Vrbjar1, Karel Frimmel1, Vladan Hudec2, Matej Ondrusek2, Ivo Gasparovic2, Rastislav Sramaty 2, Jaroslav Luptak2, Michal Hulman2, Jan Slezak1  1Centre of Experimental Medicine, Institute for Heart Research, Slovak Academy of Sciences, Bratislava, Slovakia  2Department of Cardiac Surgery, Faculty of Medicine, National Institute of Cardiovascular Diseases, Bratislava, Slovakia  Cardiac surgery-associated acute kidney injury (CS-AKI) is common postoperative complication following cardiac surgery. Since oxidative stress is hypothesized to be one of the causes of CS-AKI, molecular hydrogen (H2) supplementation has been proposed as a novel and promising antioxidant for the prevention of CS-AKI. Our study was performed on an in vivo model of simulated porcine heart transplantation. Animals in the H2 group received H2 in gaseous form (3%) during inhalation of anesthesia and throughout oxygenation of blood in extracorporeal circulation. The levels of creatinine, urea, and phosphorus were measured in plasma. Renal tissue samples were analyzed by the Western blot method (Nrf2; Keap-1; SOD) as well as by measuring enzyme kinetics of Na,K-ATPase. After cardiac surgery selected plasma biomarkers were elevated. The use of H2 was followed by the normalization of all these parameters. Our results suggest activation of Nrf2/Keap1 pathway as well as increased SOD protein expression in the H2-treated group. Regarding Na,K-ATPase we detected a significant decrease of the Vmax in the H2 group. Our results support the effectiveness of H2 supplementation in CS-AKI, especially in terms of normalization of plasma biomarkers to control levels. The protective effect of H2 might be linked to its activity against oxidative stress via Nrf2/Keap1 pathway modulation. H2 treatment resulted in decrease in renal Na,K-ATPase activity indicating decreased sodium reabsorption, which appears to be a novel regulatory mechanism of an important membrane transporter during CS-AKI in the H2-treated pig model.  Keywords: Molecular hydrogen, Cardiac surgery, Kidney injury, Oxidative stress  Funding: APVV-15-0376, APVV-19-0317, ITMS 26230120009, 2018/7838:1-26C0, 2019/4-CEMSAV1, VEGA 2/0063/18, 2/0092/22, 2/0148/22.               

Molecular hydrogen : new protective tool against acute kidney injury associated with cardiac surgery : [abstract]

http://acta.bibl.u-szeged.hu/77493/1/iacs_es_2022_031.pdf

Molecular hydrogen : new protective tool against acute kidney injury associated with cardiac surgery : [abstract]

Abstract

Similar works

Full text

Available Versions

University of Szeged