Dhanjal, Tarvinder

Edwards, David

George, Christopher

Lellouche, Nicolas

Von Ruhland, Christopher

Williams, Alan

Online Research @ Cardiff

Print this Page for Your Records Close WindowControl/Tracking Number: 16-A-8303-HRSActivity: Abstract SubmissionCurrent Date/Time: 11/30/2015 2:08:02 PMIncreased Myofibroblast densities are specific to Critical Isthmus sites in Post-Infarct Re-entrant Ventricular TachycardiaAuthor Block: Tarvinder S. Dhanjal, PhD, MRCP, Nicolas Lellouche, MD, Chris von Ruhland, PhD, David Edwards, PhD, Christopher George, PhD and Alan Williams, PhD. Wales Heart Research Institute, Cardiff, United Kingdom, Hopital Henry Mondor, creteil, France Abstract:IntroductionThe critical isthmus (CI) in post-MI re-entrant VT is a target for catheter ablation. There is compelling in vitro evidence that increased densities of myofibroblasts (MFBs) within the scar border zone (BZ) increases susceptibility to slow conduction and VT. However, the presence of MFBs within the CI in vivo remains unproven. Using an in vivo swine model of post-MI re-entrant VT we tested the hypothesis that there are significant differences in MFB distribution in the VT inducible heart compared to the post-MI VT non-inducible heart with the VT CI possessing a unique cellular profile driven by differences in MFB density.MethodsDomestic pigs (n=15) underwent MI by catheter based LAD balloon occlusion (120 min). VT studies (n=12) were performed 6 weeks post-MI identifying dense scar, BZ and the CI. Electroanatomic histological overlay was achieved with epicardial points. Hearts were formalin fixed with histological analyses performed on paraffin embedded tissue.ResultsVT was induced in 6 of 12 pigs: mean VT cycle length 353+89 ms. A total of 780 sections were analysed (867 x 687 microns per section). There was a striking 5 fold increase in MFBs at the CI compared to non-isthmus BZ sites (figure 1B). Significantly increased numbers of fibroblasts (FBs) and vessels were observed at the CI (figure 1C,D) with conduits of MFBs bridging islands of CMs (figure 1E,F).ConclusionWe have shown that the CI in vivo is characterised by a unique abundance and Page 1 of 3OASIS, The Online Abstract Submission System30/11/2015http://www.abstractsonline.com//cSubmit/SubmitPrinterFriendlyVersion.asp?Control...Leave OASIS FeedbackPowered by OASIS, The Online Abstract Submission and Invitation System SM© 1996 - 2015 Coe-Truman International, Inc. All rights reserved.architectural organisation of MFBs. This study demonstrates novel insights in the cellular composition of the CI which forms the basis for further molecular investigation which may lead to improved CI targeting for catheter ablation.:Author Disclosure Information:T.S. Dhanjal: None. N. Lellouche: None. C. von Ruhland: None. D. Edwards: None. C. George: None. A. Williams: None. Category (Complete):  08 Whole Animal Electrophysiology and Pharmacology (includes Neurohumoral Modulation) Keywords (Complete):  V -> Ventricular tachycardia ; A -> Arrhythmias - mechanism ; myofibroblasts; stromal cells Additional Information (Complete): Presentation Preference: Oral or PosterI am interested in submitting an abstract for one of the Late-Breaking Trials sessions.: NoAt the conclusion of this presentation, attendees will be able to: (Maximum character limit 250)*Learning Objective: : appreciate the cellular complexity of critical isthmus sites and understand the importance of myofibroblasts as a target for cell based therapy.Abstract Awards (Complete): The Eric N. Prystowsky Early Career Researcher Award : TrueStatus: CompletePage 2 of 3OASIS, The Online Abstract Submission System30/11/2015http://www.abstractsonline.com//cSubmit/SubmitPrinterFriendlyVersion.asp?Control...Page 3 of 3OASIS, The Online Abstract Submission System30/11/2015http://www.abstractsonline.com//cSubmit/SubmitPrinterFriendlyVersion.asp?Control...

Increased myofibroblast densities are specific to critical isthmus sites in post-infarct re-entrant ventricular tachycardia

https://orca.cardiff.ac.uk/id/eprint/91719/1/HRS%25202016%2520abstract.pdf

Increased myofibroblast densities are specific to critical isthmus sites in post-infarct re-entrant ventricular tachycardia

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