Baryscapus transversalis (Hymenoptera: Eulophidae) was established for first time as an egg hyperparasitoid of Thaumetopoea pityocampa (Lepidoptera: Notodontidae) in Bosnia and Herzegovina. It was reared from egg batches of pine processionary moth collected on Pinus nigra in the region of Boracko jezero. In laboratory conditions, 80 specimens of B. transversalis were reared during emerging period of 20 days between 3 and 22 November 2013. In the eggs of T. pityocampa, both males and females of B. transversalis developed, in sex ratio (&#9792;&#9792;:&#9794;&#9794;) 3:1.Baryscapus transversalis (Hymenoptera: Eulophidae) je determiniran kao jajni hiperparazitoid Thaumetopoea pityocampa (Lepidoptera: Notodontidae) u Bosni i Hercegovini. Uzgojen je iz jajnih legla borovog četnjaka prikupljenih s lokaliteta Boračko jezero (Konjic) i time postao nova vrsta u entomofauni Bosne i Hercegovine. U laboratorijskim uvjetima uzgojeno je 80 jedinki B. transversalis u razdoblju od 3. do 22. studenog 2013. godine. Iz jaja T. pityocampa razvili su se i mužjaci i ženke B. transversalis u odnosu (&#9792;&#9792;:&#9794;&#9794;) 3:1

Georgi Georgiev

Margarita Georgieva

Mirza Dautbasic

Osman Mujezinovic

Peter Boyadzhiev

Plamen Mirchev

English

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1 Assist. Prof. Dr Peter Boyadzhiev, Department of Zoology, University of Plovdiv, 24 Tzar Assen Str., BG-4000, Plovdiv, Bulgaria;2 Prof. Dr Mirza Dautbašić,  Assist. Prof. Dr. Osman Mujezinovic, Faculty of Forestry University of Sarajevo, Zagrebacka str. 20, Sarajevo, Bosnia-Herzegovina;3  Prof. DSc Plamen Mirchev,  Prof. DSc Georgi Georgiev,  Assist. Prof. Dr Margarita Georgieva, Forest Research Institute – Bulgarian Academy of Sciences, 132 St. Kl. Ochridski Blvd., BG-1756, Sofia, Bulgaria; E-mail: plmirchev@hotmail.comUDK 630* 442 (001) Izvorni znanstveni članak – Original scientific paperŠumarski list, 1–2 (2015): 69–71SummaryBaryscapus transversalis (Hymenoptera: Eulophidae) was established for first time as an egg hyperparasitoid of Thaumetopoea pityocampa (Lepidoptera: Notodontidae) in Bosnia and Herzegovina. It was reared from egg batches of pine processionary moth collected on Pinus nigra in the region of Boracko jezero. In laboratory conditions, 80 specimens of B. transversalis were reared during emerging period of 20 days between 3 and 22 November 2013. In the eggs of T. pityocampa, both males and females of B. transversalis developed, in sex ratio (♀♀:♂♂) 3:1.KEY WORdS: first record, hyperparasitoid, Thaumetopoea pityocampa, Bosnia and HerzegovinaBaryscapus transversalis Graham (Hymenoptera: Eulophidae) – A nEW SpECIES FOR THE FAUnA OF BOSnIA And HERZEGOvInABaryscapus transversalis Graham (Hymenoptera: Eulophidae) – NOVA VRSTA U FAUNI BOSNE I HERCEGOVINEPeter BOYADZHIEV1, Mirza DAUTBASIC2 Osman MUJEZINOVIC2, Plamen MIRCHEV3, Georgi GEORGIEV3, Margarita GEORGIEVA3tera: Encyrtidae). The meconium of B. transversalis was described for the first time by Schmidt et al. (1997).This note reports B. transversalis as a hyperparasitoid of pri-mary egg parasitoids of T. pityocampa and a new species for the fauna of Bosnia and Herzegovina.MATERIAL And METHOdSMATERIJAL I METODEThirty-eight egg batches of T. pityocampa were collected in the region of Boracko jezero (Bosnia and Herzegovina) on Pinus nigra Arnold trees at 760 m a.s.l. on 21 September 2013 by the second author. The material was mailed directly InTROdUCTIOnUVODBaryscapus transversalis Graham, 1991 (Hymenoptera: Eu-lophidae) was described on biological material from nor-thern  Greece (Graham 1991). Later, it was reported in other countries of the Balkan Peninsula (Noyes 2014), Iberian Peninsula (López-Sebastián et al. 2002–2003) and Asiatic part of Turkey (Mirchev et al. 2004). Bellin (1995) studied  its life cycle and  behaviour and established that it is an obligatory  hyperparasitoid of the primary egg para-sitoids on Thaumetopoea pityocampa (Denis & Schiffer-müller, 1775) – Baryscapus servadeii (Domenichini, 1965) and Ooencyrtus pityocampae (Mercet, 1921) (Hymenop-70  Šumarski list, 1–2, CXXXIX (2015), 69–71to the Forest Research Institute in Sofia for further obser-vations of parasitoid emergence. Every egg batches were placed singly in test tubes plugged with cotton stoppers, af-ter some measurements had been taken. Then, they were preserved in laboratory conditions at room temperature (20–22 ºC). Daily observations were made and the emerged egg parasitoids were removed immediately and separated in plastic capsules for further determination.B. transversalis was identified by the key of Graham (1991) and confirmed by Dr. E. Yegorenkova (Ulyanovsk State Pe-dagogical University, Ulyanovsk, Russia). A part of biolo-gical material is deposited in her entomological collection.RESULTSREZULTATIIn this study B. transversalis was recorded for the first time in Bosnia and Herzegovina and is therefore a new member for the fauna of this country. In laboratory conditions, 80 specimens of the species emerged from 8.515 eggs out of 38 pine processionary moth egg batches.The emerging period of B. transversalis ended in 20 days (Graph. 1), between 3 and 22 November 2013.Anastatus bifasciatus (Geoffroy, 1785) (Hymenoptera: Eu-pelmidae).As a general rule, the hyperparasitism does not increase the parasitoid impact but the effectiveness of primary parasi-toids is reduced. Therefore, the record of the hyperparasi-toid B. transversalis in Bosnia and Herzegovina is very im-portant. Percentage of B. servadeii and O. pityocampae in the host eggs attacked by the hyperparasitoid in more re-ports were from 0.5–3.0% (Tsankov et al. 1996; Schmidt et al. 1997) to 8–12 % (Mirchev et al. 1998a), and in a site in Albania reached up to 23.6% (Mirchev et al. 2000).After the first description of B. transversalis (Graham 1991), it was found again in Greece (Schmidt et al. 1997; Tsankov et al. 1999; Mirchev et al. 2010) and in other countries of Balkan Peninsula: Bulgaria (Tsankov et al. 1996, 1998; Mirchev et al. 1998a, 1998b, 2012) and Albania (Mirchev et al. 2000).Studies of the life cycle of B. transversalis show that the re-production period lasted from spring to autumn when the primary parasitoids are in diapause as mature larvae (Bellin 1995). The developmental period from egg to adult lasted about 5 weeks, and the adults did not survive more than 5 weeks. A sex ratio of 2:1 to 1:1 (females to males) was found. Males arise from arrhenotocal parthenogenesis. Tsankov et al. (1996) pointed out that B. transversalis is less successful in larvae of O. pityocampae than in those of B. servadeii.In the present study, the established flight period of B. tran-sversalis was very short –more than 50% of individuals emerged in the first day, and 80% – for three days (Graph. 1). The emerging period of other egg parasitoids of T. pityo-campa lasted more than eight and a half months from 3 November 2013 to 21 July 2014 (unpublished data).Other studies reported that B. transversalis always emerge before the mass flight of its host, the primary parasitoids, O. pityocampae and B. servadeii  (Tsankov  et  al.  1996; Schmidt et al. 1997; Mirchev et al. 2000, 2004).In most localities of B. transversalis both sexes of the species were established.  In Bulgaria,  the sex ratio varies  from about equal  numbers at Marikostino (Tsankov et al. 1998) to 10 times more of females at Ploski (Tsankov et al. 1996). Females were 2 times more in the sample from the island of Hydra (Greece) (Schmidt et al. 1997), and as the average for all surveyed sites in Turkey (Mirchev et al. 2004), in Albania – 1.2 times more (Mirchev et al. 2000). In Bulgaria in Kurtovo and Kardzhali higher parti-cipation of males was registered – 83.3% and 55.8%, respec-tively (Mirchev et al. 1998a).The present finding of B. transversalis increase the strength of entomofauna of Bosnia and Herzegovina and enhance knowledge on parasitoid complex of T. pityocampa in this country.In the eggs of T. pityocampa both males and females of B. transversalis developed. From 80 specimens emerged, 60 were females and 20 males – i.e. sex ratio (♀♀:♂♂) was 3:1.dISCUSSIOnDISKUSIJADautbašić (2015) reported three species only as egg parasi-toids of pine processionary moth in Bosnia and Herzego-vina: Baryscapus servadeii, Ooencyrtus pityocampae and Graph. 1. Emergence dynamics of Baryscapus transversalis in labora-tory conditions (N=80)Graf. 1. Dinamika pojave Baryscapus transversalis u laboratorijskim uvjetima (N=80)71BOYAdZHIEv, p. et al.: Baryscapus transversalis Graham (Hymenoptera: Eulophidae) – A NEW SPECIES FOR THE FAUNA OF BIHACKnOWLEGMEnTSZAHVALAWe thank Dr. E. Yegorenkova (Department of Zoology, Ul-yanovsk State Pedagogical University, Ulyanovsk, Russia) for her help to clarifying the taxonomic status of Barysca-pus transversalis.REFEREnCESLITERATURA•  Bellin, S. 1995: Zur Biologie von Baryscapus transversalis Gra-ham (Hym.; Eulophidae), Hyperparasitoid der Primarparasito-iden von Thaumetopoea pityocampa  (Den. & Schiff.)  (Lep.; Thaumetopoeidae), Mitteilungen der Deutschen Gesellschaft für Allgemeine und Angewandte Entomologie, 9 (4–6): 453–457. Müncheberg, Germany.• Dautbašić, M. 2015: The Pine Processionary Moth in Bosnia and Herzegovina, In: Roques, A. (ed.), Processionary Moths and Climate Change: An Update, 119–120.• Graham, M. W. R. de V. 1991: A reclassification of the European Tetrastichinae (Hymenoptera: Eulophidae): revision of the re-maining genera, Memoirs of the American Entomological In-stitute, 49: 1–322. Gainesville, USA.•  López-Sebastián, E., J. Selfa, J. Pujade-Villar, M. J. Juan-Mar-tínez, 2002–2003: Baryscapus transversalis Graham, 1991 (Hy-menoptera, Chalcidoidea, Eulophidae), nuevo hiperparasitoide asociado a la procesionaria del pino en la Penísula Ibérica, Zo-ologica Baetica, 13/14: 243–245. Granada, Spain.• Mirchev, P., G.H. Schmidt, G. Tsankov, 1998a: The egg parasi-toids of Thaumetopoea pityocampa (Den. & Schiff.) (Lep., Thau-metopoeidae) in Bulgaria,Mitteilungen aus der Biologischen Bundesanstalt  für  Land- und Forstwirtschaft,  Berlin-Dah-lem, 356: 45–52. Berlin, Germany.• Mirchev, P., G. H. Schmidt, G. Tsankov, 1998b: The egg parasi-toids of  the pine processionary moth Thaumetopoea pityo-campa (Den. & Schiff.) in the Eastern Rhodopes, Bulgaria, Bol-lettino di Zoologia Agraria e Bachicoltura, 30 (2): 131–140. Pavia, Italy.• Mirchev, P., G. H. Schmidt, G. Tsankov, S. Pllana, 2000: Egg parasitoids of the processionary moth Thaumetopoea pityo-campa (Den. & Schiff.) collected in Albania, Bollettino di Zoo-logia Agraria e di Bachicoltura (II), 31 (2): 152–165. Pavia, Italy.• Mirchev, P., G. H. Schmidt, G. Tsankov, M. Avci, 2004: Egg par-asitoids of Thaumetopoea pityocampa (Den. & Schiff.) (Lep., Thaumetopoeidae) and their impact in SW Turkey, Journal of Applied Entomology, 128 (8): 533–542. Berlin, Germany.• Mirchev, P., G. Tsankov, E. Douma-Petridou, N. Avtzis, 2010: Comparative analysis of participation of egg parasitoids of pine processionary moth, Thaumetopoea pityocampa (Den. et Schiff.) (Lep., Thaumetopoeidae) in northern and southern mainland Greece, Silva Balcanica, 11 (1): 73–97. Sofia, Bulgaria.• Mirchev, P., G. Georgiev, P. Boyadzhiev, M. Matova, 2012: Im-pact of entomophages on density of Thaumetopoea pityocampa in egg stage near Ivaylovgrad, Bulgaria, Acta zoologica bulgar-ica, Suppliment 4: 103–110. Sofia, Bulgaria.• Noyes, J. S. 2014: Universal Chalcidoidea database. World Wide Web electronic publication. http://www.nhm.ac.uk/chalcidoids. London, UK.•  Schmidt, G.H., G. Tsankov, P. Mirchev, 1997: Notes on the egg parasitoids of Thaumetopoea pityocampa (Den. and Schiff.) (In-secta: Lepidoptera: Thaumetopoeidae) collected on the Greek island Hydra, Bollettino di Zoologia Agraria e di Bachicoltura (II), 29 (1): 91–99. Pavia, Italy.•  Tsankov, G., E. Douma-Petridou, P. Mirchev, G. Georgiev, A. Koutsaftikis, 1999: Spectrum of egg parasitoids and rate of para-sitism of egg batches of the pine processionary moth Thaume-topoea pityocampa (Den. & Schiff.) in the northern Peleponnes/Greece, Journal of the Entomological Research Society, 1 (2): 1–8. Ankara, Turkey.•  Tsankov, G., G.H. Schmidt, P. Mirchev, 1996: Parasitism of egg-batches of the pine processionary moth Thaumetopoea pityo-campa (Den. & Schiff.) (Lep., Thaumetopoeidae) in various re-gions of Bulgaria,  Journal of Applied Entomology, 120 (2): 93–105. Berlin, Germany.•  Tsankov, G., G. H. Schmidt, P. Mirchev, 1998: Studies on the egg parasitism in Thaumetopoea pityocampa over a period of four years (1991–1994) at Marikostino/Bulgaria, Anzeiger für Schä-dlingskunde, Pflanzenschutz und Umweltschutz, 71 (1): 1–7. Berlin, Germany.SažetakBaryscapus transversalis (Hymenoptera: Eulophidae) je determiniran kao jajni hiperparazitoid Thaumetopoea pityocampa (Lepidoptera: Notodontidae) u Bosni i Hercegovini. Uzgojen je iz jajnih legla borovog četnjaka prikupljenih s lokaliteta Boračko jezero (Konjic) i time postao nova vrsta u entomofauni Bosne i Hercegovine. U laboratorijskim uvjetima uzgojeno je 80 jedinki B. transversalis u razdoblju od 3. do 22. studenog 2013. go-dine. Iz jaja T. pityocampa razvili su se i mužjaci i ženke B. transversalis u odnosu (♀♀:♂♂) 3:1.KLjUČnE RIjEČI: prvi nalaz, hiperparazitoid, Thaumetopoea pityocampa, Bosna i Hercegovina

Baryscapus transversalis Graham (Hymenoptera: Eulophidae) – NOVA VRSTA U FAUNI BOSNE I HERCEGOVINE\u27

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