Recently, sulphur (S) deficiencies have been observed throughout Europe (Zaoh et al., 2002). Grassland covers about 50 % of the agricultural area in the European Union. In cutting regimes exports are ca 30 kg S/ha per year. Atmospheric depositions provide, at the present, ca15 kg S/ha per year and S is not considered in fertiliser programmes. Without fertilisation, the S balance at the field scale is negative and therefore some cut swards could be S deficient. Tools for determining whether the grassland is deficient are required. The utilisation of indicators based on S content and N:S ratio was investigated by using S deficient grasses produced in a controlled environment