Blu-ray drive is an engineering masterpiece that integrates disc rotation,
pickup head translation, and three lasers in a compact and portable format.
Here we integrate a blood-coated image sensor with a modified Blu-ray drive for
high-throughput cytometric analysis of various bio-specimens. In this device,
samples are mounted on the rotating Blu-ray disc and illuminated by the
built-in lasers from the pickup head. The resulting coherent diffraction
patterns are then recorded by the blood-coated image sensor. The rich spatial
features of the blood-cell monolayer help down-modulate the object information
for sensor detection, thus forming a high-resolution computational bio-lens
with a theoretically unlimited field of view. With the acquired data, we
develop a lensless coherent diffraction imaging modality termed rotational
ptychography for image reconstruction. We show that our device can resolve the
435 nm linewidth on the resolution target and has a field of view only limited
by the size of the Blu-ray disc. To demonstrate its applications, we perform
high-throughput urinalysis by locating disease-related calcium oxalate crystals
over the entire microscope slide. We also quantify different types of cells on
a blood smear with an acquisition speed of ~10,000 cells per second. For in
vitro experiment, we monitor live bacterial cultures over the entire Petri dish
with single-cell resolution. Using biological cells as a computational lens
could enable new intriguing imaging devices for point-of-care diagnostics.
Modifying a Blu-ray drive with the blood-coated sensor further allows the
spread of high-throughput optical microscopy from well-equipped laboratories to
citizen scientists worldwide