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Giancotti, Filippo G., MD, PhD

Huse, Jason

Mondal, Jayanta

Zhang, Junfeng

English

OpenWorks @ MD Anderson

ABCFigure 1. (A) FACS analysis revealed that loss ofBRD7 led to upregulation of neutrophil infiltrationand downregulation of macrophage, dendriticcells and monocytic cells infiltration in the lung.(B) scRNAseq demonstrated that loss of BRD7 ledto upregulation of the neutrophil and the CD8+exhausted T cells infiltration in the lung. (C) GSEAof the scRNAseq data found that loss of BRD7 ledto the significant enrichment of the negativeregulation of immune system response.AB CFigure 2. (A) scRNAseq data revealed to us that loss of BRD7 led to the upregulation of the activated CD8+ exhausted T cells which was demonstrated by theupregulation of the exhausted T cell markers like CTLA4, LAG3,TIGIT, TOX and PDCD1. (B) Schematic representation of the experimental design for the scRNAseqand FACS experiments. ( C) Luminex cytokine multiplex assay revealed to us that loss of BRD7 led to the downregulation of the CCL7, CCL11, CXCL10 and CCL20cytokines in the supernatant and of the CXCL2 cytokines in the cell lysate while leading to the upregulation of the IL6 and IL33 cytokines in the cell lysate.GRAPHICAL ABSTRACT

Loss of BRD7 promotes breast cancer lung metastasis by reprogramming the tumor immune microenvironment

https://openworks.mdanderson.org/cgi/viewcontent.cgi?article=1014&amp;context=leading-edge

Loss of BRD7 promotes breast cancer lung metastasis by reprogramming the tumor immune microenvironment

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