Thirty-two soybean genotypes were assessed to estimate genetic variability parameters in RBD design with three replications during 2021. The results of the Analysis of Variance (ANOVA) revealed significant variation across the genotypes for each trait studied which showed high variability in experimental material. The higher magnitude (>20%) of GCV and PCV were exhibited by seed yield per plant (33.71% and 35.60%) followed by biological yield per plant (26.64% and 28.47%), number of pods per plant (24.56% and 25.13%) and number of branches per plant (20.24% and 20.75%), respectively. High heritability coupled with high genetic advance showed by seed yield, biological yield, number of pods, number of branches per plant, harvest index, 100-seed weight and plant height, indicating additive gene action and provides the chance for selection-based development of these traits. In this study, higher yield was reported for AUKS 213 (12.44 g) followed by AUKS 259 (11.14 g), AUKS 263 (10.82 g), AUKS 262 (10.44 g) and AUKS 264 (9.99 g) genotypes as per their mean values