Fagen, Kelly

English

MacEwan Open Journals (MacEwan University)

      Student Research Week Proceedings Vol. 1 January 2016  1  A Novel Method for the Detection of Microcystin- LR using a DNA-aptamer based Biosensor  Talk: Kelly Fagen Bachelor of Science: Biology, MacEwan University  Faculty mentor: Dr. Nina Bernstein Arts & Science: Biological Science, MacEwan University  Abstract  Cyanobacteria, or blue green algae, occurs in ponds, lakes and Slow moving streams and creates toxins known as microcystin (Ng et al. 2012). Prolonged exposure to microcystin can lead to liver damage and eventually death and causes recreational and ecological effects (Lin et al. 2012) (Eissa et al. 2014). Microcystin exists in various forms and microcystin-LR (MC-LR) is the most common and he most toxic of these structures and the one that will be the focus of our project (Ng et al. 2012). In this project, we propose the development of a novel method to detect MC-LR in water samples by using a DNA-aptamer based biosensor. We propose the use of optical devices (etalons) that will be functionalized with aptamers, DNA molecules that specifically bind to MC-LR to allow for the optical detection of the toxin in water samples (Li et al. 2015). Etalons will change their physical properties due to interactions with the resolving agent and this will alter the visual signal, % reflectance, that is recorded (Li et al. 2015). The addition of MC-LR should alter this signal in direct correlation with its concentration in the water sample. This method should prove relatively rapid and inexpensive, making it potentially superior to other methods that are currently available for the detection of MC-LR. The ability to rapidly and inexpensively measure MC-LR in water samples is highly relevant due to the high prevalence of blue green algae and toxicity of the molecule.             Student Research Week Proceedings Vol. 1 January 2016  2 References  Eissa s. Ng A. Siaj M. Zourob M. Label-free vortammetricaptasensor for the sensitive detection of microcystin-LR using graphene-modified electrodes. Analytical chemistry. 2014. 86. 7551 -7557  Lin Z. Huang H. Xu Y. Gao X. Qui B. Chen X. Chen G. Determination of microcystin-LR in water by a label-free aptamer based electrochemical impedance biosensor. Talanta. 2012. 103. 371-374  Li X. Gao Y. Serpe M. Responsive polymer-based assemblies for sensing applications. Macromolecular Rapid Communications. 2015. 36. 1382-1392  Ng A. Chinnappan R. Eissa s. Liu H. Tlili C. Zourob M. Selection, characterization, and biosensing application of high affinity congener-specific microcystin-targeting aptamers. Environmental science and Technology. 2012.46. 10697-10703 

A Novel Method for the Detection of Microcystin- LR using a DNA-aptamer based Biosensor

https://journals.macewan.ca/studentresearch/article/download/526/431

A Novel Method for the Detection of Microcystin- LR using a DNA-aptamer based Biosensor

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