Not Available60-d experimental trial was conducted to evaluate the effects of alternative feeding, restriction feeding, andrefeeding on digestive enzymes, metabolic enzymes, and metabolites in Rohu Labeo rohita ?ngerlings. Fish weighingaround 13 ? 2 g were randomly distributed into four treatment groups and a control group. The control group was fedwith 3% body mass twice a day throughout the experiment. The four treatment groups consisted of T1 (alternate dayfeeding), T2 (0.5% feeding), T3 (1% feeding), and T4 (2% feeding). The ?sh were fed for the ?rst 30 d according tothe feeding schedule, and from day 31 onwards all of the treatment groups were fed the same rations as were the con-trol ?sh up to 60 d. The samples were collected on days 0, 15, 30, 45, and 60 of the experimental trial. The activityof digestive enzymes decreased signi?cantly (P < 0.05) in T1, T2, and T3 with respect to the control, and the lowestactivities were found on day 30 of the experiment. The glucose-6-phospahate dehydrogenase activity decreased withthe level of restriction feeding, with the lowest activity occurring at day 30 in the T2 group, and it improved withrefeeding. The serum glucose, triglycerides, and cholesterol concentrations decreased, with the lowest levels occurringin the T2 group at day 30. The current study revealed that restriction feeding and refeeding affected the digestion andmetabolism of Rohu ?ngerlings within a short period of 30 d, and refeeding restored the optimum activity levels withinthe same 30-d period thereafte
