Introduction: Urinary tract infection (UTI) is one of the most common infections in humans, accounting for more than 150 million cases worldwide. Beyond the initial urinary infection, catheter-associated UTIs (CAUTIs) can lead to complications including bacteremia, endocarditis, osteomyelitis, septic arthritis, and meningitis. With this background, the current study was designed to provide baseline information of such infections in the hospital and to identify the microbial pathogens associated with these infections. Methodology: A cross-sectional study was carried by the Department of Microbiology, Jan Nayak Karpuri Thakur Medical College and Hospital, Bihar, from January 2021 to July 2021. Both adult males and females were included in the study group. Before starting the study, the purpose of the study was explained to the subjects, and informed consent was obtained. Approval was acquired from the Ethical Committee. Results: The present study comprised of 150 catheterized urine samples collected with proper aseptic precautions. Mean age of the participating patients was 47.5 years with a standard deviation of 7.2 years. The age range was 17 years to 81 years. The male to female ratio was calculated and approximated to 1.5:1. The incidence rate of CAUTI calculated for this study for ICU and wards were 3.7 and 17.2 per 1000 catheter-days, respectively and catheter utilization ratio was 0.59 and 0.07 in ICU and ward, respectively. Conclusion: The study underscores the pressing need for the development of antimicrobial urinary catheters and their deployment when longer duration of catheter access is required