ABSTRACTObjective: The aim of the study is evaluation of toxicity assessment of biosynthesized silver nanoparticles from Solanum villosum (Mill.) using in vitro and in vivo study.Materials and Methods: Biologically synthesized silver nanoparticles are characterized by UV, SEM, EDAX, XRD analysis and its cytotoxicity effect against HepG2cell lines was performed. Further toxicity was confirmed by in vivo studies using wistar albino rats. Various hematological, liver function marker enzymes and liver histopathology are investigated.The cytotoxic effect of Solanum villosum silver nanoparticles (SV-AgNPs) was also concentration dependent and did not produce any toxicity to tested animals. The histopathological evidence are supported to biochemical observations.Conclusion: So biologically synthesized silver nanoparticles are toxic only to cancer cells but not in animals were proved by present study. Key Words: SV-AgNPs (Solanum villosum silver nanoparticles), HepG2 cell lines, Scanning electron microscopy (SEM), energy dispersive x-ray spectroscopy (EDAX), X-ray diffraction analysis (XRD).Â
