ABSTRACTObjective: The aim of this study is to explore the protective effect of non-irradiated and γ-irradiated mango juice (NMJ and GMJ) against ethanolinducedhepatic and renaldysfunctionin rats.Methods: A total of 36 male Wistar rats were selected and randomly divided into six groups of six animals each, and the treatment was given everyday via orogastric tube for 30 days. Group I rats served as normal control group. Group II, III, IV, V, and VI rats received absolute ethanol, NMJ, GMJ,ethanol+NMJ, and ethanol+GMJ, respectively. At the end of experimental period, the animals were sacrificed, and serum was separated for liverand kidney markers analysis. The tissues (liver and kidney) were excised for histopathological changes and lipid peroxidation, enzymatic, and nonenzymaticantioxidantsactivitieswereestimated.Results: Ethanol treatment resulted in the depletion of superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, glutathione-stransferaseactivities,andreducedvitaminCandreducedglutathionecontent,higherlevelofmalondialdehyde,increasedactivitiesofserumalanineaminotransferase,aspartateaminotransferase,alkalinephosphatase,gamma-glutamyltranspeptidase and elevated levels of serum urea, creatinine,uric acid causing malfunction of hepatic, and renal tissues, when compared to control rats. Mango juice (both NMJ and GMJ) co-treatment with ethanolingested rats, were envisaged to possess significant antioxidant defense properties and help in the recovery of tissues from ethanol-induced oxidativedamage. The results showed that degenerative changes in hepatic and renal cells of ethanolic groups were minimized by the administration of mangojuice, as revealed by histopathological examination.Conclusion: The current findings suggest that treatment with mango juice reduces ethanol-induced oxidative stress, thereby protecting the hepaticand renal tissue from ethanol-induced damage.Keywords: γ-irradiation, Mango juice, Ethanol, Oxidative stress, Antioxidant enzymes, Liver, Kidney
