ABSTRACTObjective: Helicteres isora Linn. roots have been reported to use in microbial infection, diabetes, dysentery colic, scabies, wound healing, andgastropathy. The main objective of this study was preliminary phytochemical screening of various fractions of H. isora roots.Methods: In this study, pharmacognostic and phytochemical investigations on the various fractions of H. isora roots were carried out with thestandard procedures.Results: The pharmacognostic analysis revealed moisture content (0.18%), total ash (4%), acid insoluble ash (1%), and water soluble ash (1.5%). Thequalitative phytochemical screening revealed the presence of alkaloids, amino acids, proteins, carbohydrates, flavonoids, cardiac glycosides, saponin,steroids, terpenoids, and tannins.Conclusion: H. isora roots are a rich source of various biological active constituents. H. isora may be further investigated for the isolation of bioactivecompound that could be developed into drug for the treatment of various human diseases.Keywords: Helicteres isora, Pharmacognostic, Phytochemical, Roots.Â