Comparison of ELISA and ELISPOT methods in measuring CD4+ T cell responses to a newly identified MHC class II-restricted epitope within Simian Virus 40 T antigen
ELISA and ELI SPOT were used to monitor CD4+ and CD8+ responses to SV 40 Tag MHC class I and class II-restricted epitopes. It was found that freezing and thawing IFN-y samples decreased their ability to be detected by ELISA. Therefore, Femto-HS ELISA reagents were necessary to detect weaker CD4+ responses when using frozen supernatants; responses detected were comparable to those detected by ELISPOT. Later experiments using direct capture ELISA, ELISA of fresh supernatants and ELIS POT showed that the CD4+ responses were stronger when MHC class I-restricted epitopes were absent from the SV40 T ag
