The aplication of Lean Thinking due to decrease losings and increase local organization has been gradually growing in many areas beyond industrial sector and one of those that this thought started to afford lots of benefits is the health sector. This article it´s about a case study that sought to align 5S tool of Lean Methodology in health sector with the objective of bring improvements to the local study process and pitch in advances of Lean HealthCare theme. The study environment chosen is the pharmacy of hemodialysis sector of a hospital located in the east center of Minas Gerais, Brazil, and through the aplication of a 5S audit form it was executed a qualitative-quantitative study. From the quantification of 5S level existant in the pharmacy before and after the 5S program implementation, it was possible to offer as a result improvements proposal to work environment and employees, in addition to recommended actions to keep the program active