Protective Effect of Vitamin E on Indomethacin Induced Gastric Ulcers

Abstract

Background: To study the histomorphological changes in gastric mucosa induced in mice by Indomethacin and to assess the protective effect of vitamin E with variable duration of drugs.Methods: In this experimental study 48 adult albino mice were divided randomly in to four groups A, B, C and D each group comprising of 12 mice. Group A was the control group. Group B was given Vitamin E 400mg/kg. Group C was given Indomethacin 25mg/kg and Group D was given a combination of Indomethacin and Vitamin E (25mg/kg & 400 mg/kg respectively). In each group 6 animals were given dose for 3 days and other 6 animals for 8 days. They were sacrificed 24 hours after the last dose. After dissection gastric mucosal changes regarding colour and site of ulcers were examined under dissecting microscope. Histological parameter observed was presence/absence of mucus in the cells of gastric glands.Results: Findings were normal in groups A and B. Reddish mucosa with ulcers in pyloric part of stomach was seen in groups C and D. Decreased mucus in cells of gastric glands was also observed in group C as compared to group D.Conclusion: Severity of gastric ulcers induced by Indomethacin in mice is decreased by giving Vitamin E which protects gastric mucosa by increasing mucus in cells of gastric glands