In the present study, organosolv pretreatment strategy was modified for the comprehensive utilisation of spent coffee waste (SCW) for polyphenol extraction and reducing sugar production. A response surface design involving four factors viz. ethanol concentration, temperature, time and H2SO4 concentration was created for optimisation of polyphenol extraction from SCW. 11.2 mg GAE of polyphenol content/g of SCW was obtained using ethanol (68% v/v) at 51 °C for 45 min in the presence of 1.5% H2SO4 as catalyst. Furthermore, 13.78 of QE of Total Flavonoid Content/g of SCW was obtained following this process which was 7.6-fold higher than control (1.81 QE/g of SCW). Lignin removal of 24.4% was achieved following modified organosolv protocol. Enzymatic hydrolysis of pretreated SCW yielded 29.05 mg/g of total reducing sugar, which was 1.27-fold higher than control (22.8 mg/g). XRD, FTIR, and DSC profiles of the pretreated substrates were analysed for various changes incurred in pretreated SCW
