In peach, pollen sterility, expressed as absence of pollen in the anthers, segregates as an undesired trait in breeding programs. Pollen fertility screening in progenies is not a common practice mainly because it does not affect fruit set since cross-pollination is frequent. It is also a time-consuming activity that coincides with the busy pollination season. Segregation for this trait could be avoided by using molecular markers to identify appropriate parents or male sterile plants for early culling in progenies expected to segregate, thus increasing breeding efficiency. In peach, pollen sterility is determined by a recessive allele in homozygosis of the major gene, Ps/ps, located on chromosome 6. In this work, using a conventional mapping approach combined with bulked segregant analysis using resequencing data, we fine mapped Ps to a region of almost 160 kb and developed molecular markers for marker-assisted breeding. These markers were validated in plant materials from three peach breeding programs, including progenies, advanced selections, and cultivars, allowing us to determine that the frequency of the ps allele is high (0.23) and also to infer the genotypes of a large collection of cultivars and advanced breeding lines.info:eu-repo/semantics/acceptedVersio
