Pengendalian Penyakit Layu Bakteri Nilam Menggunakan Pseudomonad Fluoresen

Abstract

Controlling bacterial wilt disease on patchouli plant withfluorescent pseudomonadThe study of controlling bacterial wilt disease on patchouli plant(Ralstonia solanacearum) with fluorescent pseudomonad was carried outin a farmer's field in Situak Village West Pasaman, West Sumatera fromOctober 2003 to June 2004. The aims of the study were to find out theeffectiveness of fluorescent pseudomonad for controlling bacterial wiltdisease, increasing plant growth and production. Isolates of fluorescentpseudomonad Pf 63, Pf 90, Pf 91, Pf 147 and Pf 180 as treatments wereisolated from the rhizosphere of healthy patchouli plant, and selectedbased on antagonistic activity on R. solanacearum in vitro at theLaboratory of Plant Bacteriology, Faculty of Agriculture, UGM. Theisolates were inoculated on patchouli plant and adapted for one weekbefore planting. The plants treated with fluorescent pseudomonadisolates were planted in the field infected with pathogen on October2003. The experiment was arranged in a randomized block design(RBD) with six replications. The assessment parameters were incubationperiod, disease intensity, plant growth and production of patchouli plants.The results showed that fluorescent pseudomonad isolates could controlthe bacterial wilt disease and delay the incubation period 6-52 days anddecrease the disease intensity 31,11–50,56%. In addition fluorescentpseudomonad isolates could affect the increase of plant growth, i.e. plantheight ( 6,7 – 26,3 cm ), leaf numbers (4,6 – 30,1 leaves/plant) and dryweight of leaves (24,5 – 154,3 g/plant), and plant production, especiallyoil content (4,8 – 22,3 ml/plant). The results of the experiment showedthat Pf 91 isolate had the highest antagonistic activity on controlling thebacterial wilt disease on field