This research intend to detect the best of grow medium composition and Rootone-F concentration towards growth of nyatoh stump on nursery. This research was conducted on March to May 2015. This research consisting two factors of grow medium (M) and Rootone-F concentration that comprise of four levels so it would contain 16 treatments. Each treatment consist of five groups, so here would be 80 nyatoh stumps. The parameter that observed are high accretion, leaf total, stump diameter accretion, wet weight of root and shoot, dry weight of root and shoot, and seeds quality index. Experimental technique was used Randomized Complete Block Design with Factorial Treatment. The result showed that treatment interaction significant affected to high accretion, leaf total, stump diameter accretion, wet weight of root and shoot, dry weight of root and shoot, and seeds quality index. The conclusions of research is soil growing medium composition: manure (2:1) with 100 ppm Rootone-F (M2R1) give the best treatment to nyatoh stump growth on nursery. Soil growing medium composition: manure (2:1) with 100 ppm Rootone-F (M2R1) have high of seeds quality index value so it will most feasiblef cultivated for meet the availability of quality seeds