Pemanfaatan Teknik Kriopreservasi Dalam Penyimpanan Plasma Nutfah Tanaman

Abstract

The two basic approaches for conservation of plant genetic resources are ex situ and in situ conservation. Cryopreservation is a potential method for long term preservation of plant germplasm. Cryopreservation technique could be used to preserve plant materials with unlimited time (until 20 years) because it bring the materials to metabolically inactive state and completely arrest the growth in liquid nitrogen at –196oC. This method is more efficient in terms of cost, time, space, and labour because it does not require frequenlty subculture. Cryopreservation techniques can be divided in to classical technique and new techniques. Classical technique can be applied to limited species but new techniques can be applied to wide range of species and many types of explant. The success of cryopreservation is not only indicated by the high rate of survival and regenerated culture, but also on the stability level of culture after cryopreservation